Attachment 1 Purchase Description.pdf

Version 11-30-2016-1

PURCHASE DESCRIPTION

1. Statement of Need and Purpose:

The purpose of this acquisition is procurement of automated, UV and visible light protein crystallization imaging system for inspection and detection of protein crystals in crystallization droplets.

2. Background Information and Objective:

The Cell Biology and Biophysics Unit/ NINDS conducts biophysical and structural studies on microtubules and microtubules regulating proteins with focus on understanding the function of post-translational modifications on the recruitment of cellular effectors. The dual mode visible light and UV-fluorescence microscope will be used to support ongoing crystallization trial of microtubules related proteins. The microscope provides the way for: (1) detection of protein crystals in crystallization droplets in trays and automatic imaging of multiple crystallization conditions (2) discrimination between protein and salt crystals by comparison of visible, UV and fluorescence images, (3) detection of microcrystals hidden at the edges of the droplets or masked by amorphous precipitate, (4) selection of potential crystallization hits leading to diffracting crystals.

3. Generic Name of Product:

Automated, visible light and UV fluorescence microscope for protein crystals detection.

4. Purchase Description:

(1) Trade name: N/A

(2) Product’s brand name: UVEX-P brightfield/UV fluorescence protein crystals inspection automated compound microscope for SBS and Linbro plates

(3) Manufacturer's name: JAN Scientific, Inc.

(4) Model, type, catalog, and/or part number(s): UVEX-P Microscope with XPCOL

Option.

5. Salient characteristics The vendor shall provide equipment brand name or equal to the Jan Scientific Inc.

UVEX-P Microscope with XPCOL option.

Microscope must have/meet the following requirements:

(1) Reuse 5x, 15x & 40x objectives in existing microscope

(2) Dual mode bride-field and UV-fluorescence imaging option

(3) Color birefringence – crossed polarizer imaging

(4) Compatible crystallization plates type: 96-well SCS handing drops plate, 24-well

Linbro.

(5) Automatic scanning of 96-well plate

(6) Compact and light weight that the imager can fit in the available laboratory space (maximum width 16”) and can be moved into a cold room

(7) Optical magnification range: 5x to 40x

(8) Cooled monochrome 6 Mpixel camera with high UV sensitivity and color birefringence option

(9) LED light sources, low maintenance and long lasting with no IR emission

(10) UV optimized optics for low-intensity excitation and high contrast detection of fluorescence

(11) Proprietary design that allows brightfield and fluorescence images to share the same optical path resulting in images that are superimposable without software manipulation

(12) Excitation and emission wavelengths tuned for detecting the presence of and to some extent Tyr

(13) A resolution of 0.2 micron/pixel with 40x objective

(14) Can be incorporated with plate hotels at a later date

(15) Windows 10 PC with control software and image management software

(16) Installation and on-site training

(17) Minimum 12 months warranty from date of installation

6. Quantity: One (1) Microscope system

7. Delivery:

The contractor shall deliver and install the equipment within three months after receipt of order. The delivery must be FOB destination and inside laboratory. The installation and delivery of the equipment shall be between the hours of 8:00am and 5:00 pm, Bethesda, Maryland local prevailing time.