D Mod_1_75N95022Q00080.pdf

Title: Protein Crystallization and X-Ray Crystallography on Large tumor suppressor kinase 1

(LATS1)

SAM.GOV: https://sam.gov/opp/c12bc91621f84fa5a705d0c5b16b5427/view

RFQ: 75N95022Q00080

AMENDMENT No.: 01 AMENDMENT DATE: January 19, 2022 QUOTATION DUE DATE: 09:00am Eastern Time on January 20, 2022 (Unchanged)

ISSUED BY:

National Center for Advancing Translational Sciences, NIH NIDA Office of Acquisition, NCATS Section

BG 11601 RM 03C09 MSC 6012

11601 LANDSDOWN ST

BETHESDA MD 20852-6012

PRIMARY POINT OF CONTACT:

Renato Gomes, Contract Specialist Phone: (301) 451-2596 Email: renato.gomes@nih.gov

TO ALL OFFERORS:

The above-numbered solicitation is amended as set forth below. The hour and date specified for receipt of quotes REMAINS UNCHANGED.

Quoters must acknowledge receipt of this amendment on each copy of the quote submitted OR by separate letter or electronic communication which includes a reference to the solicitation and amendment numbers.

FAILURE OF YOUR ACKNOWLEDGMENT TO BE RECEIVED AT THE PLACE DESIGNATED

FOR THE RECEIPT OF QUOTES PRIOR TO THE HOUR AND DATE SPECIFIED MAY

RESULT IN REJECTION OF YOUR QUOTE.

If by virtue of this amendment you desire to change a quote already submitted, such change may be made by letter, provided each letter makes reference to the solicitation and this amendment, and is received prior to the time and date specified for receipt of quotes.

PURPOSE: respond to formal questions submitted in response to the solicitation.

All other terms and conditions of the Combined Synopsis/Solicitation remain unchanged.

RESPONSES TO QUESTIONS

The Government has provided answers below. Note that many questions may have been combined, paraphrased, and/or summarized for brevity and clarity.

1. Question: Please confirm the amount of constructs on the SOW. Are five (5) constructs required?

Answer: Phase 1 requires the contractor to design five (5) different constructs for large tumor suppressor kinase 1 (LATS1) and at least one (1) construct for any LATS1-

RFQ: 75N95022Q00080

AMENDMENT No.: 01 AMENDMENT DATE: January 19, 2022 QUOTATION DUE DATE: 09:00am Eastern Time on January 20, 2022 (Unchanged) associated proteins and express them in E. coli for suitable expression levels evaluation.

Thus, the contractor shall propose five (5) constructs on Phase 1 for NCATS evaluation.

For Phase 2, only two (2) constructs shall be selected by NCATS and expressed by the Contractor. Please refer to Attachment 1, SOW – Specific Requirements, Phase 1.

Construct Design.

2. Question: What would be the associated protein required by NCATS?

Answer: Phase 1 states that even though some of the constructs may not be the full length LATS1 protein, they must all contain the LATS1 kinase domain (IE, the ATP pocket). LATS1 may be associated with/interact with one or more different proteins that require co-expression with the LATS1 construct in order to obtain sufficient quality crystals. After award, further discussions will take place with regarding the best protein to co-express with LATS1 in order to potentially obtain sufficient quality crystals. Please refer to Attachment 1, SOW – Specific Requirements, Phase 1. Construct Design.

3. Question: In FAR 52.204-24 and FAR 52.204-26, [company’s] offer has nothing to do with telecommunication, so nothing to fill in this form except the representation that we do not provide and will not use telecommunication?

Answer: As per combined synopsis solicitation 75N95022Q00080, if applicable, the FAR Provision Representations and Certifications FAR 52.204-24 and FAR 52.204-26 must be completed, signed, and returned by the quoters with its quote pursuant to FAR

4.2103 Procedures.

4. Question: In FAR 52.225-4, what items are we expected to detail?

Answer: In accordance with the definition of the term supplies on the FAR 52.225-4 Buy American-Free Trade Agreements-Israeli Trade Act Certificate (Feb 2021), the electronic reports to be submitted as a result of the performance of the services are considered supplies.

“Supplies means the end items furnished by the Contractor and related services required under this contract. Except when this contract includes the clause entitled Warranty of Data, supplies also mean "data." (FAR 52.225-4 (Feb 2021)).

As per combined synopsis solicitation 75N95022Q00080, Attachment 6 - FAR 52.212-5 (Jan 2022), FAR 52.225-4, Alternate III (Feb 2021) applies. If applicable, the quoter shall certify that the supplies are Free Trade Agreement country end products or Israeli end products as defined in the clause of this solicitation entitled "Buy American-Free Trade Agreements Israeli Trade Act. For Free Trade Agreement country list, please refer to FAR 52.225-3 (Nov 2021).