Sol_140G0224Q0137_Amd_0002.pdf

(x)

140G0224Q0137 x x copies of the amendment; (b) By acknowledging receipt of this amendment on each copy of the offer submitted ; or (c) By separate letter or electronic communication which includes a reference to the solicitation and amendment numbers. FAILURE OF YOUR ACKNOWLEDGEMENT TO BE

RECEIVED AT THE PLACE DESIGNATED FOR THE RECEIPT OF OFFERS PRIOR TO THE HOUR AND DATE SPECIFIED MAY RESULT IN REJECTION OF YOUR

OFFER. If by virtue of this amendment you desire to change an offer already submitted , such change may be made by letter or electronic communication, provided each letter or electronic communication makes reference to the solicitation and this amendment, and is received prior to the opening hour and date specified.

x

GDB

DENVER CO 80225-0046

PO BOX 25046

USGS OAG DENVER ACQUISITION BRANCH

GDB

DENVER CO 80225-0046

204 DENVER FEDERAL CENTER

PO BOX 25046

USGS OAG DENVER ACQUISITION BRANCH

07/11/20240002

13. THIS ITEM ONLY APPLIES TO MODIFICATION OF CONTRACTS/ORDERS. IT MODIFIES THE CONTRACT/ORDER NO. AS DESCRIBED IN ITEM 14.

12. ACCOUNTING AND APPROPRIATION DATA (If required) is not extended.is extended, Items 8 and 15, and returning

Offers must acknowledge receipt of this amendment prior to the hour and date specified in the solicitation or as amended , by one of the following methods: (a) By completing

The above numbered solicitation is amended as set forth in Item 14. The hour and date specified for receipt of Offers

11. THIS ITEM ONLY APPLIES TO AMENDMENTS OF SOLICITATIONS

FACILITY CODE CODE

10B. DATED (SEE ITEM 13)

10A. MODIFICATION OF CONTRACT/ORDER NO.

9B. DATED (SEE ITEM 11)

9A. AMENDMENT OF SOLICITATION NO.

CODE

8. NAME AND ADDRESS OF CONTRACTOR (No., street, county, State and ZIP Code)

7. ADMINISTERED BY (If other than Item 6)CODE 6. ISSUED BY

PAGE OF PAGES

4. REQUISITION/PURCHASE REQ. NO.3. EFFECTIVE DATE2. AMENDMENT/MODIFICATION NO. 5. PROJECT NO. (If applicable)

1. CONTRACT ID CODE

AMENDMENT OF SOLICITATION/MODIFICATION OF CONTRACT

07/02/2024

CHECK ONE A. THIS CHANGE ORDER IS ISSUED PURSUANT TO: (Specify authority) THE CHANGES SET FORTH IN ITEM 14 ARE MADE IN THE CONTRACT

B. THE ABOVE NUMBERED CONTRACT/ORDER IS MODIFIED TO REFLECT THE ADMINISTRATIVE CHANGES (such as changes in paying office, C. THIS SUPPLEMENTAL AGREEMENT IS ENTERED INTO PURSUANT TO AUTHORITY OF:

D. OTHER (Specify type of modification and authority) appropriation data, etc.) SET FORTH IN ITEM 14, PURSUANT TO THE AUTHORITY OF FAR 43.103(b).

E. IMPORTANT: Contractor is not is required to sign this document and return __________________ copies to the issuing office.

ORDER NO. IN ITEM 10A.

14. DESCRIPTION OF AMENDMENT/MODIFICATION (Organized by UCF section headings, including solicitation/contract subject matter where feasible.)

A. The purpose of this amendment is to share questions and answers with all interested parties. The following information is hereby incorporated into the solicitation and resulting contract:

1. Question: As the SOW asks for sequencing-only and no bioinformatics, we were assuming all you required were FASTQ files. Is this correct, or do you need deliverables in addition to FASTQ files? If the latter, can you let us know what additional deliverables you require?

Government Response: No other deliverables, just FASTQ sequence files.

2. Question: Will the government be sending the libraries for the contractor to

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16A. NAME AND TITLE OF CONTRACTING OFFICER (Type or print)15A. NAME AND TITLE OF SIGNER (Type or print)

15C. DATE SIGNED 16B. UNITED STATES OF AMERICA 15B. CONTRACTOR/OFFEROR 16C. DATE SIGNED

(Signature of person authorized to sign) (Signature of Contracting Officer)

Jason Harris

STANDARD FORM 30 (REV. 11/2016)

Prescribed by GSA FAR (48 CFR) 53.243

Previous edition unusable

Except as provided herein, all terms and conditions of the document referenced in Item 9 A or 10A, as heretofore changed, remains unchanged and in full force and effect .

ITEM NO. SUPPLIES/SERVICES QUANTITY UNIT UNIT PRICE AMOUNT

NAME OF OFFEROR OR CONTRACTOR

2 3

CONTINUATION SHEET

REFERENCE NO. OF DOCUMENT BEING CONTINUED PAGE OF

(A) (B) (C) (D) (E) (F)

140G0224Q0137/0002 pool/multiplex? or will they send as one sample tube pool of 96 libraries?

Government Response: We will pool the libraries into a single tube, unless the vendor wishes to do it themselves in order to have more flexibility in the flow cell allocation.

3. Question: If the group with the USGS Technical

Liaison plans to send pools to be sequenced across 8 lanes of 10B in one project/one shipment, then we would offer discounted pricing.

However, if they are sending less than 8 lanes in one project/one shipment, the pricing will be different. This is applicable to the 10B sequencing as it is mentioned to be 12 lanes on

10B in the SOW. I'd like to provide the best pricing to the USGS technical liaison so if the group can send the pools to be sequenced across 8 lanes (one flow cell) in one shipment/on project, then those 8 lanes will be at lower rate.

Government Response: The lane numbers in the SOW were simply examples of how the requirement could be met for various instruments. If the vendor wants to use a full flow-cell (=8 lanes, and would typically be the most economical), then that is fine/preferable. They are free to tell us how many libraries to send at a given time and how they should be pooled, recognizing that we are preparing libraries in plates using Illumina

UD 96-sample index kits A, B, and C.

4. Question: How many lanes will you be sending at one time?

Government Response: we can send them to suit the vendor's availability. We don't send 'lanes', we send 96x library pools which they can sequences on as many lanes as they wish to achieve the result. Also, to clarify, the unit of samples is a plate (=pool) of 96 indexed libraries, of which we will ship at least two and at most three, depending on the cost. We do not ship 'lanes', rather the provider chooses the instrument and lane number to allocate so that we get the yield we are requiring (~20X according to the statement of work). In theory, the two or three sample pools could be sequenced with different instrument combinations to produce the desired

Continued ...

NSN 7540-01-152-8067 OPTIONAL FORM 336 (4-86)

Sponsored by GSA

FAR (48 CFR) 53.110

ITEM NO. SUPPLIES/SERVICES QUANTITY UNIT UNIT PRICE AMOUNT

NAME OF OFFEROR OR CONTRACTOR

3 3

CONTINUATION SHEET

REFERENCE NO. OF DOCUMENT BEING CONTINUED PAGE OF

(A) (B) (C) (D) (E) (F)

140G0224Q0137/0002 result.

5. Question: Will the samples be sent (12 lanes or 5 lanes) as one shipment? Or is there a plan on the timeline in which these will be shipped

(e.g. 5 of 12 lanes shipped first, then 7 of 12 lanes shipped at a later date)?

Government Response: The logistics of the shipments are negotiable and should not affect the price in a way that I am aware of (we pay the shipping costs). We can prepare the libraries to suit a given schedule, within the constraint that they have a shelf life of approximately one month. So we cannot make them until we have an agreement in place, but we can ship them together or at different times as requested by the vendor.

B. The Quotation Submission response due date hereby remains unchanged for July 16, 2024 by

12:00 PM (MDT).

Period of Performance: 07/29/2024 to 09/30/2024

Award Type: Firm Fixed Price

USGS POC: Jason Harris, jharris@usgs.gov, (303)

236-9316

NSN 7540-01-152-8067 OPTIONAL FORM 336 (4-86)

Sponsored by GSA

FAR (48 CFR) 53.110