Sol_140G0224Q0137_Amd_0001.pdf

(x)

140G0224Q0137 x x copies of the amendment; (b) By acknowledging receipt of this amendment on each copy of the offer submitted ; or (c) By separate letter or electronic communication which includes a reference to the solicitation and amendment numbers. FAILURE OF YOUR ACKNOWLEDGEMENT TO BE

RECEIVED AT THE PLACE DESIGNATED FOR THE RECEIPT OF OFFERS PRIOR TO THE HOUR AND DATE SPECIFIED MAY RESULT IN REJECTION OF YOUR

OFFER. If by virtue of this amendment you desire to change an offer already submitted , such change may be made by letter or electronic communication, provided each letter or electronic communication makes reference to the solicitation and this amendment, and is received prior to the opening hour and date specified.

x

GDB

DENVER CO 80225-0046

PO BOX 25046

USGS OAG DENVER ACQUISITION BRANCH

GDB

DENVER CO 80225-0046

204 DENVER FEDERAL CENTER

PO BOX 25046

USGS OAG DENVER ACQUISITION BRANCH

07/03/20240001

13. THIS ITEM ONLY APPLIES TO MODIFICATION OF CONTRACTS/ORDERS. IT MODIFIES THE CONTRACT/ORDER NO. AS DESCRIBED IN ITEM 14.

12. ACCOUNTING AND APPROPRIATION DATA (If required) is not extended.is extended, Items 8 and 15, and returning

Offers must acknowledge receipt of this amendment prior to the hour and date specified in the solicitation or as amended , by one of the following methods: (a) By completing

The above numbered solicitation is amended as set forth in Item 14. The hour and date specified for receipt of Offers

11. THIS ITEM ONLY APPLIES TO AMENDMENTS OF SOLICITATIONS

FACILITY CODE CODE

10B. DATED (SEE ITEM 13)

10A. MODIFICATION OF CONTRACT/ORDER NO.

9B. DATED (SEE ITEM 11)

9A. AMENDMENT OF SOLICITATION NO.

CODE

8. NAME AND ADDRESS OF CONTRACTOR (No., street, county, State and ZIP Code)

7. ADMINISTERED BY (If other than Item 6)CODE 6. ISSUED BY

PAGE OF PAGES

4. REQUISITION/PURCHASE REQ. NO.3. EFFECTIVE DATE2. AMENDMENT/MODIFICATION NO. 5. PROJECT NO. (If applicable)

1. CONTRACT ID CODE

AMENDMENT OF SOLICITATION/MODIFICATION OF CONTRACT

07/02/2024

CHECK ONE A. THIS CHANGE ORDER IS ISSUED PURSUANT TO: (Specify authority) THE CHANGES SET FORTH IN ITEM 14 ARE MADE IN THE CONTRACT

B. THE ABOVE NUMBERED CONTRACT/ORDER IS MODIFIED TO REFLECT THE ADMINISTRATIVE CHANGES (such as changes in paying office, C. THIS SUPPLEMENTAL AGREEMENT IS ENTERED INTO PURSUANT TO AUTHORITY OF:

D. OTHER (Specify type of modification and authority) appropriation data, etc.) SET FORTH IN ITEM 14, PURSUANT TO THE AUTHORITY OF FAR 43.103(b).

E. IMPORTANT: Contractor is not is required to sign this document and return __________________ copies to the issuing office.

ORDER NO. IN ITEM 10A.

14. DESCRIPTION OF AMENDMENT/MODIFICATION (Organized by UCF section headings, including solicitation/contract subject matter where feasible.)

A. The purpose of this amendment is to share questions and answers with all interested parties. This information is hereby incorporated by reference into the solicitation and resulting contract:

1. Question: Within the quality control plan section of the SOW, it's mentioned that the

QCP will need to be adhered to while performing "phytoplankton sample ID services"... can you please clarify what "phytoplankton sample ID services" means.... Is this a separate service you will need in addition to NovaSeq X sequencing?

Government Response: The "phytoplankton sample ID services" language was erroneously included in SOW under the Quality Control Section so please disregard that language. The

Continued ...

16A. NAME AND TITLE OF CONTRACTING OFFICER (Type or print)15A. NAME AND TITLE OF SIGNER (Type or print)

15C. DATE SIGNED 16B. UNITED STATES OF AMERICA 15B. CONTRACTOR/OFFEROR 16C. DATE SIGNED

(Signature of person authorized to sign) (Signature of Contracting Officer)

Jason Harris

STANDARD FORM 30 (REV. 11/2016)

Prescribed by GSA FAR (48 CFR) 53.243

Previous edition unusable

Except as provided herein, all terms and conditions of the document referenced in Item 9 A or 10A, as heretofore changed, remains unchanged and in full force and effect .

ITEM NO. SUPPLIES/SERVICES QUANTITY UNIT UNIT PRICE AMOUNT

NAME OF OFFEROR OR CONTRACTOR

2 3

CONTINUATION SHEET

REFERENCE NO. OF DOCUMENT BEING CONTINUED PAGE OF

(A) (B) (C) (D) (E) (F)

140G0224Q0137/0001 correct intended language is specifically for the

NovaSeq x Sequencing services. The Quality

Control Section of SOW is hereby updated to strike erroneous language "phytoplankton sample

ID services" and replaced with correct language for "NovaSeq x Sequencing" services.

2. Question: Will you need 2 separate quotes for both the minimum 192 samples and maximum 288 samples?

Government Response: Yes, if that is Contractor's preference. Or a quote per plate (set of 96).

3. Question: Can you please confirm that the indexes you use will result in all the libraries having their own unique index/barcode? If so, can the pools of 96 libraries be run on the same lane?

Government Response: Yes, I can confirm that each plate of 96 libraries has a unique set of 96 barcodes applied, and that the sets used on each plate are different. Specifically, we have/are using Illumina UD indexes set A, B, and C.

4. Is the Illumina NovaSeq X Plus platform acceptable as well, as long as the configuration is still 10B or 25B? The NovaSeq X Plus system is capable of dual flow cell runs, while the NovaSeq

X system is only capable of single flow cell runs.

Government Response: Yes, the NovaSeqX and

NovaSeqX Plus are equivalent for our purposes.

Also for additional clarification, we refer to each set of 96 libraries as a plate, as that is how they are prepared and stored, but we will pool each plate into a single tube and quantify prior to shipping.

B. The Question Cutoff date remains unchanged for

July 11, 2024 by 12:00 PM (MDT).

C. The Quotation Submission response due date hereby remains unchanged for July 16, 2024 by

12:00 PM (MDT).

Period of Performance: 07/29/2024 to 09/30/2024

Award Type: Firm Fixed Price

Continued ...

NSN 7540-01-152-8067 OPTIONAL FORM 336 (4-86)

Sponsored by GSA

FAR (48 CFR) 53.110

ITEM NO. SUPPLIES/SERVICES QUANTITY UNIT UNIT PRICE AMOUNT

NAME OF OFFEROR OR CONTRACTOR

3 3

CONTINUATION SHEET

REFERENCE NO. OF DOCUMENT BEING CONTINUED PAGE OF

(A) (B) (C) (D) (E) (F)

140G0224Q0137/0001

USGS POC: Jason Harris, jharris@usgs.gov, (303)

236-9316

NSN 7540-01-152-8067 OPTIONAL FORM 336 (4-86)

Sponsored by GSA

FAR (48 CFR) 53.110