Project Grant RF1AG072167

Award Date 5/1/22
Completion Date 4/30/25
Dollars Obligated $2.6M
Federal Grant Program
93.866
Assistance Type
Project Grant
Place of Performance
New York, NY 10032, USA
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Columbia University will establish mixed in vitro co-cultures of neurons, astrocytes, and microglia differentiated from iPSCs representing donors with varying AD pathology and cognitive trajectories. Preliminary data shows concordance between in vitro and post-mortem data from donor monocultures. The mixed cultures will help discern cell type-specific changes driven autonomously versus induced non-autonomously through interactions. Researchers will profile differentiated cells using single-nucleus RNA sequencing and conduct functional assays to further understand convergent cell-type effects in AD. A $2,567,371 subaward to Brigham and Women's Hospital will support all in vitro experiments, differentiations, assays, and contributions from co-Principal Investigator Dr. Tracy Young-Pearse.

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