This Project Grant award from the National Cancer Institute (CFDA 93.396 - Cancer Biology Research) will support the development of a novel approach to detect and report on the expression of multiple cytokine genes simultaneously in tissues. The $234,919 award to Yale University, to be completed by August 31, 2027, will combine CRISPR-mediated transcriptional activation with genetically-encoded fluorescent reporters to enable the dynamic, high-sensitivity and multiplexed monitoring of cytokine expression. If successful, this system could provide valuable tools and reagents to study the tumor microenvironment and immune responses, with potential applications in functional genomics and in vivo research. The project aims to address limitations of current gene expression measurement techniques by enabling continuous, spatially-resolved detection of low-abundance transcripts like cytokines, which play important roles in shaping anti-tumor immunity.
Mod # | Description | Reason For Modification | Federal Obligation (Click to sort descending) | Date (Click to sort ascending) |
|---|---|---|---|---|
| Not listed | $234.9k | 8/29/24 |