Project Grant R56HG013092

Award Date 8/9/23
Completion Date 7/31/24
Dollars Obligated $400K
Federal Grant Program
93.172
Assistance Type
Project Grant
Place of Performance
Seattle, WA 98195, USA
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The National Human Genome Research Institute (NHGRI) awarded a $400,000 Project Grant (CFDA 93.172 - Human Genome Research) to the University of Washington to develop new single-cell multi-omic technologies for studying the relationship between three-dimensional genome organization and gene expression.

Specifically, the grant will fund the development of CARE-Seq, a novel method to jointly measure whole-genome chromatin interactions and gene expression in the same single cells from complex tissues. The project will also create a new approach to concurrently profile single-cell transcriptomes and specific promoter-enhancer loops in thousands of individual cells. Additionally, the grant will combine spatial biology techniques with the single-cell methods to decipher the in situ dynamics of 3D genome structure and gene expression at single-cell resolution in tissue samples. These new technologies are expected to provide unprecedented opportunities to understand the interplay between genome architecture and transcriptional regulation across diverse biological contexts.

The University of Washington is the prime awardee, and has issued a $80,000 sub-award to Carnegie Mellon University to contribute expertise and resources to the project.

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