Project Grant R44GM152984
- This Project Grant award from the National Human Genome Research Institute (NHGRI), under the Human Genome Research federal grant program (CFDA 93.172), will provide $215,088 to Directseq Biosciences, Inc. to develop an exhaustive de novo RNA sequencing method using next-generation mass spectrometry (NGMS-seq). The objective is to create a technology that can directly identify and sequence every RNA species in a sample, while also comprehensively profiling all RNA modifications, without bias....
- This $302,671 federal Project Grant awarded on August 2, 2025 by the National Institute of General Medical Sciences (CFDA 93.859 Biomedical Research and Research Training program) to Andson Biotech Inc. aims to develop an innovative real-time mass spectrometry platform for in-process monitoring and cell state analysis of advanced cell therapies. The project seeks to address the challenges of variable quality and efficacy in cell therapies by enabling real-time measurement and control of critical...
- This $316,825 Project Grant award from the National Institute of General Medical Sciences (NIGMS), under the Biomedical Research and Research Training program (CFDA 93.859), aims to advance the field of native mass spectrometry (MS) analysis. The research project will develop an innovative intact mass deconvolution method to significantly improve the speed, accuracy, and user-friendliness of proteins and nucleic acid molecular weight determination using MS. The key objectives are to implement...
- This federal Project Grant award from the National Human Genome Research Institute (NHGRI), under the Human Genome Research CFDA program (93.172), provides $406,500 to Directseq Biosciences, Inc. to advance next-generation mass spectrometry-based sequencing (NGMS-SEQ) methods. The primary objectives are to 1) develop high-throughput NGMS-SEQ techniques for direct sequencing of transfer RNA (tRNA) samples, including physiologically relevant ones, and 2) enhance technologies to quantitatively...
- This Project Grant award from the National Institute of General Medical Sciences (NIGMS) under the Biomedical Research and Research Training program (CFDA 93.859) provides $350,000 to Electronic Biosciences, Inc. to develop the first technology capable of high-accuracy sequencing of the RNA modifications pseudouridine (Ps) and N6-methyladenosine (M6A) using nanopore sequencing. The project aims to merge innovative bisulfite adduct methodology with Electronic Biosciences' enzyme-free, multipass...
- This federal Project Grant award of $350,000 from the National Institute of General Medical Sciences (NIGMS) Biomedical Research and Research Training Program (CFDA 93.859) supports the development of a neural network-accelerated DNA and RNA sequence processing node, called CLEDGESEQ, for use in field, clinical, and industrial settings. The project aims to create advanced sequence analysis and communication capabilities for edge applications, enabling cost-effective and scalable DNA/RNA...
- This $254,182 Project Grant awarded by the National Institute of General Medical Sciences (NIGMS), under the Biomedical Research and Research Training program (CFDA 93.859), will fund the development of a novel, unified X-ray/cryo-EM pipeline for ensemble-based structural refinement. The project aims to address the significant challenges posed by structural disorder, flexibility, and conformational heterogeneity in modern therapeutic modalities, which limit the accuracy of traditional refinement...
- This Cooperative Agreement award from the Department of Health and Human Services' National Institutes of Health (NIH) under the Trans-NIH Research Support program (CFDA 93.310) supports the development of an integrated pipeline for assessing variant-associated off-target risks in genome editing therapeutics. The $856,513 award will fund the optimization and integration of two complementary approaches - the CRISPRME computational tool for nominating off-target sites based on genetic variants,...
- This Project Grant award, funded by the National Institute of General Medical Sciences (NIGMS) under the Biomedical Research and Research Training program (CFDA 93.859), supports the development and commercialization of Newomics Inc.'s UNIESI system with M3 emitters and MEA chips as an integrated imaging-mass spectrometry front-end platform for native spray mass spectrometry. The $1,000,000 award will be used to validate the platform's performance for native MS studies of biomolecular...
- This Project Grant award from the National Institute of General Medical Sciences (NIGMS) Biomedical Research and Research Training Program (CFDA 93.859) supports the development of a novel in-line biomanufacturing process analytical technology tool. The $302,417 award to Physical Sciences Inc. (PSI), in collaboration with the University of Massachusetts Lowell (UML), aims to enable real-time monitoring of biomolecules in large-scale bioreactors for gene therapy manufacturing. The technology...
ACCELERATING GENE THERAPY AND EDITING WITH ADVANCED MS-BASED DATA ANALYSIS FOR NONSTANDARD AND HYBRID NUCLEOTIDE SEQUENCES - PROJECT SUMMARY / ABSTRACT TITLE: ACCELERATING GENE THERAPY AND EDITING WITH ADVANCED MS-BASED DATA ANALYSIS FOR NONSTANDARD AND HYBRID NUCLEOTIDE SEQUENCES THIS PROJECT SEEKS TO IMPROVE RELIABILITY AND SPEED UP THE DEVELOPMENT OF LIFE-SAVING AND LIFE-ENHANCING OLIGONUCLEOTIDE-BASED THERAPEUTICS AND MAGNIFY THE POSITIVE IMPACT OF BIOMEDICAL RESEARCH AND EDUCATION WORLDWIDE, LEADING TO A QUANTUM LEAP IN OUR UNDERSTANDING OF THE MOLECULAR AND CELLULAR PATHWAYS AND MECHANISMS INVOLVED IN HEALTHY AND DISEASED BIOLOGICAL SYSTEMS. DIRECT SEQUENCING VIA HIGH-RESOLUTION MASS SPECTROMETRY IS AN ESSENTIAL ASSAY IN THE DEVELOPMENT AND QA/ MANUFACTURING OF THESE THERAPEUTICS BUT MANUAL INTERVENTION IN THE DATA ANALYSIS PIPELINE DUE TO INADEQUATE SOFTWARE HAS CAUSED A BOTTLENECK IN THE PROCESS OF GETTING THESE LIFE-SAVING THERAPEUTICS TO THOSE IN NEED. SIMILARLY, IN-DEPTH CHARACTERIZATION OF RNA AND THEIR MODIFICATIONS NEEDED FOR IMPROVING BIOMARKER IDENTIFICATION IS HINDERED IN THE RNA RESEARCH COMMUNITY. TO ADDRESS THESE CHALLENGES, SEMI-AUTOMATED DIRECT SEQUENCING OF OLIGONUCLEOTIDES BY MASS SPECTROMETRY WILL BE IMPLEMENTED AND DELIVERED ON MASSMATRIX'S HIGH-THROUGHPUT, ENTERPRISE CLOUD PLATFORM (MMCLOUD). ENHANCEMENTS TO MASSMATRIX'S OLIGONUCLEOTIDE SEQUENCING ENGINE WILL BE MADE FOR CHARACTERIZING NONSTANDARD, HYBRID AND CONJUGATE OLIGONUCLEOTIDES. A UNIFIED DATA REPOSITORY ON MMCLOUD WILL ENABLE ASSAY COMPARISONS, KNOWLEDGE CAPTURE AND MANAGEMENT, AND COLLABORATION WITHIN AND ACROSS RESEARCH TEAMS. TOGETHER, THESE AIMS ARE EXPECTED TO IMPROVE THE RELIABILITY AND ACCURACY OF RESULTS AS WELL AS TO SIGNIFICANTLY REDUCE THE OLIGONUCLEOTIDE CHARACTERIZATION BOTTLENECK FOR THE PHARMACEUTICAL INDUSTRY AND THE RESEARCH COMMUNITY. DEEPER UNDERSTANDING AND BETTER DECISION MAKING WILL FOLLOW, BOTH HAVING A POTENTIALLY DRAMATIC POSITIVE IMPACT ON DOWNSTREAM PROCESSES AND RESOURCE DEPLOYMENT, INCLUDING IMPROVED DRUG SAFETY, EFFICACY AND AVAILABILITY OF LIFE- SAVING AND ENHANCING THERAPEUTICS.
Mod # | Description | ReasonForModification | Federal Obligation | Date |
|---|---|---|---|---|
| Not listed | $0 | 8/4/25 | ||
| Not listed | $860.6k | 7/1/24 | ||
| Not listed | $860.6k | 7/5/23 | ||
| Not listed | $860.6k | 7/5/23 |