This federal Project Grant award from the National Institute on Aging (CFDA 93.866 - Aging Research) provides $1,274,632.00 to Epicypher, Inc. to develop CUTANA-RH, a novel technology platform for genome-wide profiling of R-loops - triple-stranded nucleic acid structures strongly associated with aging and neurodegenerative diseases. The key products and services to be delivered include: A modular immunotethering strategy using a catalytically inactive RNase H1 enzyme as a detection reagent for...
This $500,000 Project Grant awarded by the National Institute on Aging (CFDA 93.866 Aging Research) will support Sxrna Technologies, Inc. in developing an organoid toolkit for Alzheimer's disease (AD) and Alzheimer's-disease-related dementias (ADRD). The company aims to create a platform for identifying senescent cells in living tissue, which can provide insights into the aging process and enable high-throughput drug screening in complex 3D tissue models. Specifically, the grant will fund...
This federal Project Grant award from the National Institute on Aging (CFDA 93.866 - Aging Research) in the amount of $619,681 to The Leland Stanford Junior University will support the development of "Combo-Seq/Tag", a toolkit combining gene editing and protein tagging technologies. The goal is to efficiently perturb Alzheimer's disease (AD) risk genes and conduct multi-omics analyses in human stem cell and in vivo mouse models. The award will yield innovative gene editing vectors...
This $448,961 Project Grant award from the National Institute on Aging's Aging Research program (CFDA 93.866) aims to develop a low-cost, accurate, and high-throughput diagnostic test for telomere-associated diseases. The proposed test will use a DNA array and fluorescent in situ hybridization (FISH) to measure the absolute lengths of individual telomeres, providing a detailed telomere length profile. This innovative approach could enhance the diagnosis and management of diseases linked to...
This federal Project Grant award for $506,404.00 was provided by the National Institute on Aging (NIA) under the Aging Research program (CFDA 93.866). The award supports research by the University of Pennsylvania to investigate mechanisms of local and global epigenetic changes that drive senescence and aging-associated phenotypes. Key focus areas include: 1) understanding how enhancers are rewired during senescence and aging, 2) investigating how cryptic transcription is initiated within genes...
This $459,525 Project Grant awarded by the National Institute on Aging (CFDA 93.866 - Aging Research) to Aperture Therapeutics, Inc. aims to rapidly discover a novel oligonucleotide therapeutic for direct modulation of a genetically validated target involved in pathologic neuroinflammation. The project leverages Aperture Therapeutics' high-throughput induced pluripotent stem cell (iPSC) platform with diverse genetic models of frontotemporal dementia (FTD) to provide robust functional evidence...
This $1,737,000 Project Grant awarded by the National Institute of General Medical Sciences (NIGMS) under the Biomedical Research and Research Training program (CFDA 93.859) supports the development of innovative single-cell genomics technologies for joint analysis of regulatory dynamics and transcriptional states. Key objectives include: Developing multiomics tools to measure rates of epigenomic changes (DNA methylation, demethylation, oxidative damage, DNA repair) and relate these to...
This National Institute on Aging R01 Project Grant Award (CFDA 93.866 - Aging Research) provides $745,092 to the University of Virginia to conduct research on the epitranscriptomic mechanisms underlying the pathogenesis of Alzheimer's disease (AD) and related tauopathies. The key objectives are to: 1) Decode the epitranscriptomic profiles impacted by tau pathology using m6A DART-seq, 2) Determine the role of RNA methylation in AD pathogenesis using 3D neuron-glial brain assembloids, and 3)...
This federal Project Grant award for $660,908.00 was provided by the National Institute on Aging (NIA) under the Aging Research program (CFDA 93.866) to Brandeis University. The award will fund research to uncover the functions of circular RNAs (circRNAs) in the aging process, particularly in the central nervous system. Key objectives include determining the mechanisms behind circRNAs that promote aging, examining the role of circRNA translation in aging, and defining the relationship between...
This Project Grant from the National Institutes of Health's National Institute on Aging, under the Aging Research program (CFDA 93.866), provides $4,041,438 to develop new computational tools and biomarkers for Alzheimer's disease. The University of Miami will aggregate and analyze DNA methylation datasets to identify age-associated epigenetic changes related to aging and Alzheimer's progression. Researchers will create a searchable web interface to disseminate findings on the role of DNA...
This $1,274,993 Project Grant award from the National Institute on Aging (CFDA 93.866 - Aging Research) will fund the development of Epicypher's Reverse Transcribe & Tagmentation (RT & TAG) technology. RT & TAG is a new genomic approach to identify chromatin-associated RNAs (CARNAs) including nascent RNA transcripts, which are critical for understanding aging and age-related diseases like Alzheimer's. The central innovation is an immunotethering-based method for in situ targeted tagmentation of CARNAs. Epicypher will also develop novel spike-in controls to enable assay optimization, performance monitoring, and standardized data normalization. In Phase I, Epicypher successfully applied RT & TAG to identify histone PTM-associated polyadenylated RNAs. In Phase II, they will optimize RT & TAG for analyzing polyadenylated CARNAs, extend the method to capture nascent and non-polyadenylated transcripts, and integrate user-friendly bioinformatics pipelines to prepare for commercial launch. This transformative technology will enable new research on the links between CARNAs, epigenetic dysregulation, and aberrant gene expression in aging and age-related diseases.