A portable quantitative polymerase chain reaction platform (qpcr) for rapid detection of pathogens impacting model organisms in animal facilities
Ended Project Grant Awarded Last action
- Award ID
- R43OD034167
- Recipient
-
Bay Genomics, LLC UEI DNE2JJ6LEK28
- Awarding agency
-
Aggregate Financial Assistance Data
National Institutes of Health, Department of Health and Human Services
- Obligated
- $509,980
Award facts
- Assistance listing
- 93.351 Research Infrastructure Programs
- Completion date
- Originally
- Funding agency
- Office of the Director National Institutes of Health, Department of Health and Human Services
- Place of performance
- California, USA
Award details come from USAspending.gov.
Description
- Abstract
- In recent years there have been growing concerns about the rigor and reliability of data originating from common and critical animal model experimentation. In fact, a core principal of science - reproducibility - has been called into question largely due to challenges in replicating biologically complicated animal experiments. We have developed a fast, portable, quantitative PCR (the current universal "gold standard" method) device for use in a wide variety of diagnostic settings. We believe that our rapid, handheld, battery-powered and wireless device has the potential to empower rapid diagnostics around the world. In this proposal we aim to validate the use of our prototype device using real-world samples for microbial pathogen detection. Regarding our innovative technology, we have taken a fundamentally different approach to heating and cooling the sample being amplified, avoiding the standard peltier heating block. The heat transfer is so efficient that we are able to perform a heating and cooling cycle in as little as 15 seconds (possibly even faster), resulting in a 40-cycle run finishing in about 11 minutes. In essence, this means you can get a quantitative answer regarding the presence and abundance of your target pathogen with ultimate sensitivity significantly faster than the current state of the art. Looking deeper into the root causes of animal-derived data variability is pointing to several possible environmental and biological causes. In terms of the biology, microbes that live inside or outside of the body are proving to have clear and present effects on experimental outcomes, yielding data that is inconsistent and questionable. In particular, when the studies of interest are translational by design, and therefore intended to benefit / improve human health, there is even greater cause for concern. We are poised to test our device to identify and quantify the presence (or absence) of pathogens in an fully operating academic animal facility at UC berkeley. Together with the office of laboratory animal care, we have identified two important and tractable pathogens as good candidates for initial proof of principle studies: helicobacter species (bacteria) in mice and b. Dendrobatidis, a fungus affecting amphibians.
Transactions
| Action | Obligated | Date |
|---|---|---|
| Amendment | -$32 | |
| Amendment | $0 | |
| Amendment | $0 | |
| Amendment | $255,006 | |
| Original award | $255,006 |
Quick answers
Who received award R43OD034167?
R43OD034167 was awarded to Bay Genomics, LLC by Aggregate Financial Assistance Data (National Institutes of Health) on August 1, 2023.
How much is award R43OD034167 worth?
$509,980 has been obligated on R43OD034167.
Which agency awarded R43OD034167?
Aggregate Financial Assistance Data (National Institutes of Health) awarded R43OD034167, with funding from Office of the Director (National Institutes of Health).
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