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All Federal Grant Awards
Project Grant R43HG010592
Award Date
9/1/19
Completion Date
2/28/21
Dollars Obligated
$350K
Overview
Activity
3
Transactions
3
Subawards
Similar Awards
Federal Agency
National Human Genome Research Institute
Awardee
Scribe Biosciences, Inc. (QMCJSMLF9TP1)
Federal Grant Program
93.172
Assistance Type
Project Grant
Place of Performance
California, USA
Update #1
Update #2
A PLATFORM FOR INTEGRATING CELL SIGNALING AND SINGLE CELL TRANSCRIPTOMICS.
Posted 8/20/19
5
1
Mod #
Description
ReasonForModification
Federal Obligation
Date
Not listed
A PLATFORM FOR INTEGRATING CELL SIGNALING AND SINGLE CELL TRANSCRIPTOMICS. - PROJECT SUMMARY CELLS COMMUNICATE THROUGH PHYSICAL INTERACTION OF CELL SURFACE PROTEINS OR THROUGH AUTOCRINE, PARACRINE, JUXTACRINE OR ENDOCRINE MECHANISMS. HOWEVER, CELL TYPES IDENTIFIED BY CURRENT LARGE-SCALE SINGLE-CELL SEQUENCING AND SPATIAL TRANSCRIPTOMICS EFFORTS MAY MASK HETEROGENEITY IN FUNCTIONAL RESPONSES BETWEEN CELL TYPES. OUR ULTIMATE VISION FOR A PRODUCT WOULD BE A PLATFORM TO INTERROGATE MULTIPLE CELL-TYPES IN DIFFERENT COMBINATIONS AND UNDER DIFFERENT BIOCHEMICAL CONDITIONS TO UNDERSTAND HETEROGENOUS GENE EXPRESSION RESPONSES. IN PHASE I OF THIS SBIR, SCRIBE BIOSCIENCES WILL ADAPT ITS PROPRIETARY PRINTED DROPLET MICROFLUIDICS (PDM) TECHNOLOGY TO STUDY THE IMPACT OF PERTURBATIONS ON SINGLE-CELL TRANSCRIPTOMICS. PDM CAN PRECISELY DELIVER REAGENTS AND SINGLE CELLS TO A MICROWELL ARRAY AT ANY TIME, ENABLING FLEXIBLE MULTI-STEP WORKFLOWS WHILE MAINTAINING HIGH EXPERIMENTAL THROUGHPUTS. PDM WILL BE USED TO ENCAPSULATE SINGLE-CELLS, INCUBATE THEM EITHER WITH AN AGONIST/ANTAGONIST OR ADDITIONAL CELL(S) OF DIFFERENT CELL-TYPES, AND PERFORM SCRNA-SEQ TO UNDERSTAND INDUCED TRANSCRIPTIONAL CHANGES. A NOVEL LIPID-BARCODING SCHEME WILL BE USED TO LINK CELLS ENCAPSULATED IN THE SAME DROPLETS, ENABLING CELLS TO BE RECOVERED WITH TRACEABLE SIGNATURES IN BULK AND SEQUENCED BY ESTABLISHED SINGLE- CELL METHODS. IN SPECIFIC AIM 1 WE WILL DEMONSTRATE THE ABILITY TO PERTURB AND INCUBATE SINGLE CELLS IN A TEST SYSTEM WHERE SINGLE JURKAT CELLS ISOLATED IN DROPLETS ARE TREATED WITH ANTI-CD3 AND ANTI-CD28 ANTIBODIES, THEN ASSAYED FOR IL2P EXPRESSION. IN SPECIFIC AIM 2 WE WILL DEMONSTRATE A BARCODING METHOD TO BIOINFORMATICALLY LINK CELLS INCUBATED WITHIN THE SAME DROPLET WHILE ALLOWING THEM TO BE SEQUENCED SEPARATELY. THE SUCCESSFUL EXECUTION OF THIS PHASE I PROGRAM WILL DEMONSTRATE THE TECHNICAL FEASIBILITY OF A HIGH THROUGHPUT FUNCTIONAL GENOMICS PLATFORM USING OUR PDM TECHNOLOGY AND WILL SET THE STAGE FOR A NUMERICAL SCALE UP OF THE PLATFORM TO ANALYZE UP TO 10,000 SINGLE CELLS.
$0
12/22/21
Not listed
A PLATFORM FOR INTEGRATING CELL SIGNALING AND SINGLE CELL TRANSCRIPTOMICS. - PROJECT SUMMARY CELLS COMMUNICATE THROUGH PHYSICAL INTERACTION OF CELL SURFACE PROTEINS OR THROUGH AUTOCRINE, PARACRINE, JUXTACRINE OR ENDOCRINE MECHANISMS. HOWEVER, CELL TYPES IDENTIFIED BY CURRENT LARGE-SCALE SINGLE-CELL SEQUENCING AND SPATIAL TRANSCRIPTOMICS EFFORTS MAY MASK HETEROGENEITY IN FUNCTIONAL RESPONSES BETWEEN CELL TYPES. OUR ULTIMATE VISION FOR A PRODUCT WOULD BE A PLATFORM TO INTERROGATE MULTIPLE CELL-TYPES IN DIFFERENT COMBINATIONS AND UNDER DIFFERENT BIOCHEMICAL CONDITIONS TO UNDERSTAND HETEROGENOUS GENE EXPRESSION RESPONSES. IN PHASE I OF THIS SBIR, SCRIBE BIOSCIENCES WILL ADAPT ITS PROPRIETARY PRINTED DROPLET MICROFLUIDICS (PDM) TECHNOLOGY TO STUDY THE IMPACT OF PERTURBATIONS ON SINGLE-CELL TRANSCRIPTOMICS. PDM CAN PRECISELY DELIVER REAGENTS AND SINGLE CELLS TO A MICROWELL ARRAY AT ANY TIME, ENABLING FLEXIBLE MULTI-STEP WORKFLOWS WHILE MAINTAINING HIGH EXPERIMENTAL THROUGHPUTS. PDM WILL BE USED TO ENCAPSULATE SINGLE-CELLS, INCUBATE THEM EITHER WITH AN AGONIST/ANTAGONIST OR ADDITIONAL CELL(S) OF DIFFERENT CELL-TYPES, AND PERFORM SCRNA-SEQ TO UNDERSTAND INDUCED TRANSCRIPTIONAL CHANGES. A NOVEL LIPID-BARCODING SCHEME WILL BE USED TO LINK CELLS ENCAPSULATED IN THE SAME DROPLETS, ENABLING CELLS TO BE RECOVERED WITH TRACEABLE SIGNATURES IN BULK AND SEQUENCED BY ESTABLISHED SINGLE- CELL METHODS. IN SPECIFIC AIM 1 WE WILL DEMONSTRATE THE ABILITY TO PERTURB AND INCUBATE SINGLE CELLS IN A TEST SYSTEM WHERE SINGLE JURKAT CELLS ISOLATED IN DROPLETS ARE TREATED WITH ANTI-CD3 AND ANTI-CD28 ANTIBODIES, THEN ASSAYED FOR IL2P EXPRESSION. IN SPECIFIC AIM 2 WE WILL DEMONSTRATE A BARCODING METHOD TO BIOINFORMATICALLY LINK CELLS INCUBATED WITHIN THE SAME DROPLET WHILE ALLOWING THEM TO BE SEQUENCED SEPARATELY. THE SUCCESSFUL EXECUTION OF THIS PHASE I PROGRAM WILL DEMONSTRATE THE TECHNICAL FEASIBILITY OF A HIGH THROUGHPUT FUNCTIONAL GENOMICS PLATFORM USING OUR PDM TECHNOLOGY AND WILL SET THE STAGE FOR A NUMERICAL SCALE UP OF THE PLATFORM TO ANALYZE UP TO 10,000 SINGLE CELLS.
$0
12/22/21
Not listed
A PLATFORM FOR INTEGRATING CELL SIGNALING AND SINGLE CELL TRANSCRIPTOMICS.
$350.0k
8/20/19