Project Grant R43HG008562

This Project Grant award from the National Institutes of Health's National Human Genome Research Institute provided $768,678 to Molecular Assemblies, Inc. to develop a fully enzymatic oligonucleotide synthesis cycle using engineered template-independent polymerases and a novel phosphate dNTP blocking group. The award supports the goals of the Human Genome Research program (CFDA 93.172) to accelerate genome research and its application to human health. Specifically, the awardee will engineer...

The National Institute of General Medical Sciences (NIGMS) awarded a $305,892 Project Grant under the Biomedical Research and Research Training program (CFDA 93.859) to Dotquant LLC, a biotechnology research and development company, to develop a new in vitro transcription kit for high-yield synthesis of chemically modified RNA. The project aims to combine the benefits of wild-type T7 RNA polymerase, which has high efficiency and low cost, with the ability of mutant T7 RNA polymerases to...

The National Institute of General Medical Sciences (NIGMS) awarded Epicypher, Inc. a $1,264,176 Project Grant under the Biomedical Research and Research Training program (CFDA 93.859) to develop a novel manufacturing method for asymmetric designer nucleosomes (A-DNucs). The goal is to advance the study of the "histone code" - the complex signaling mechanisms by which post-translational modifications on histones regulate chromatin function and gene expression. The technical innovation...

This federal Project Grant award of $444,262.00, provided by the National Institute of General Medical Sciences (NIGMS) under the Biomedical Research and Research Training program (CFDA 93.859), supports the development of new reagents and methods for synthesizing oligophosphate constructs. The key products and services to be delivered include: Developing new reagents and methods for synthesizing oligophosphate chains up to pentaphosphate and beyond, which can be used to enable the exploration...

This $349,746 Project Grant awarded by the National Center for Advancing Translational Sciences (NCATS) under CFDA Program 93.350 aims to develop an innovative platform for synthesizing high-quality, purified mRNA with minimal contaminants such as double-stranded RNA, long RNA, and short truncated RNA. The goal is to create an mRNA production technology that can address issues with impurities in current methods, which can provoke immune responses and compromise the safety and efficacy of...

This Project Grant award from the National Institute of General Medical Sciences (NIGMS), under the Biomedical Research and Research Training program (CFDA 93.859), provides $350,000 in funding to Electronic Biosciences, Inc. to develop advanced nanopore-based sequencing technology capable of accurately and concurrently detecting the RNA modifications pseudouridine (Ps) and N6-methyladenosine (M6A). The project aims to overcome limitations of current sequencing methods by merging Electronic...

This $256,000 National Science Foundation Engineering grant supports the development of an easy RNA adenylation method for deep sequencing of picogram RNA samples with high resolution. Specifically, Ezrabio Inc. will develop a versatile and cost-effective library construction technology that enables protein translation monitoring on a variety of biological samples. The proposed technology relies on a novel enzyme system capable of 5' adenylation and 3' polyadenylation of RNA fragments to...

This Project Grant award from the National Center for Advancing Translational Sciences (NCATS), a component of the National Institutes of Health (NIH), aims to develop recombinant human enzyme panels representing genetic variants in drug-metabolizing enzymes that are present in the U.S. patient population. The $285,444 award to Precision Quantomics, Inc. will leverage recombinant proteins to create commercially viable kits for polymorphic drug-metabolizing enzymes that exhibit variability....

This Project Grant award from the National Center for Advancing Translational Sciences (NCATS), part of the National Institutes of Health (NIH), is funding the development of a high-throughput kinetic system for small-molecule drug discovery. The $329,272 award to Capienda Biotech, LLC will be used to upgrade an existing prototype instrument to enable rapid characterization of small-molecule compounds that target human cyclin-dependent kinases, which are important therapeutic targets for...

This $3,000,000 project grant, awarded by the National Science Foundation (NSF) under the Engineering (CFDA 47.041) program, aims to solve the long-standing challenge of developing self-amplifying ribonucleic acid (SARNA) technologies. The project, led by Trustees of Boston University, a private non-profit research university, will: Synthesize a diverse library of modified nucleoside triphosphates (ModNTPs) and use them to produce modified SARNA with extended cellular half-life. Evaluate the...