Project Grant R43GM146542
- This National Science Foundation (NSF) Project Grant award for $380,074, awarded on September 1, 2024, aims to develop a platform for instrument-free, easy-to-interpret quantitative analysis of molecular biomarkers. The project will produce a general methodology adaptable to a range of molecular targets, including potential new agents. The key products to be delivered include: A "yes/no" output for quantitative measurement, enabled through stoichiometry and negative cooperativity-based...
- The federal Project Grant award of $350,000 from the National Institute of General Medical Sciences (NIGMS) under the Biomedical Research and Research Training program (CFDA 93.859) is supporting the development and demonstration of a novel enzyme-based, single-molecule protein sequencing system by Electronic Biosciences, Inc. This innovative platform leverages enzyme-driven processing and sequencing of individual proteins and peptides, combined with advanced bioinformatics, to achieve high...
- This Project Grant award from the National Institute of General Medical Sciences (NIGMS) Biomedical Research and Research Training Program (CFDA 93.859) supports the development of a novel in-line biomanufacturing process analytical technology tool. The $302,417 award to Physical Sciences Inc. (PSI), in collaboration with the University of Massachusetts Lowell (UML), aims to enable real-time monitoring of biomolecules in large-scale bioreactors for gene therapy manufacturing. The technology...
- The National Human Genome Research Institute (NHGRI), under the Federal Grant Program "Human Genome Research" (CFDA 93.172), awarded a $407,291 Phase I Small Business Innovation Research (SBIR) grant to Electronic Biosciences, Inc. to develop a nanopore-based protein sequencing platform. The project aims to create a high-accuracy, label-free nanopore reader capable of direct, single-molecule protein sequencing. This novel technology would enable proteome-wide profiling, biomarker...
- This $275,000 Small Business Innovation Research (SBIR) Phase I award from the National Science Foundation (NSF) Technology, Innovation, and Partnerships (TIP) program supports the development of a label-free, high-resolution, high-throughput protein screening platform called Vibrational Spectroscopy with Metasurface Optics (VISMO). The technology aims to enable simultaneous, minute-scale measurement of millions of protein samples, which would dramatically improve the lengthy and costly cycles...
- This Project Grant award from the National Institute of General Medical Sciences (CFDA 93.859 - Biomedical Research and Research Training) to Kasa Bio, L.L.C., a woman-owned small business, aims to develop a higher-order multiplex digital PCR (dPCR) method for detecting important gene fusions related to chronic myeloid leukemia. The $345,285 award, with a performance period from May 1, 2025 to April 30, 2026, will fund the development of Kasa Bio's proprietary "ZIP-Melt" multiplex...
- The National Science Foundation Division of Chemical, Bioengineering, Environmental, and Transport Systems awarded $800,000 under the Engineering (47.041) federal grant program to develop a novel biosynthetic toolkit for rapid detection and monitoring of COVID-19 antibodies. Funded from August 1, 2022 through July 31, 2025, Indiana University will construct SARS-CoV-2 epitope peptides fused with a nanotag transducer peptide and displayed on an RNA phage platform. This biosynthetic display aims...
- This National Science Foundation (NSF) Technology, Innovation, and Partnerships (CFDA 47.084) program award provides $274,990 to Dencoda LLC to develop a novel DNA-based kinase assay platform. The key products to be delivered are: A highly sensitive, specific, and multiplexed kinase activity assay that can be used as a research tool to discover new therapeutic targets or as a clinical diagnostic test for diseases involving kinase dysregulation, such as cancers, diabetes, and Alzheimer's disease....
- The National Institute of General Medical Sciences (NIGMS) awarded a $306,872 Project Grant under the Biomedical Research and Research Training program (CFDA 93.859) to Fluid Discovery, Inc., a San Francisco-based biotechnology company, to develop a "Universal Platform for Ultrahigh-Throughput Biocatalyst Evolution with Micro-Mass Spectrometry." The award supports the development of a commercial prototype for Fluid Discovery's novel screening platform, which combines scalable hardware,...
- Micropure Genomics Inc. received a $274,199 Project Grant award from the National Science Foundation to advance methods for rapid, end-to-end sample preparation for sequencing applications. Under the NSF Technology, Innovation, and Partnerships program (CFDA 47.084), the company will develop an automated process relying on electro-hydrodynamic trapping to isolate genomic material like DNA or RNA from cells in a flow cartridge. The process aims to significantly reduce preparation time while...
DNA-BASED ASSAY FOR CALORIMETRIC DETERMINATION AND QUANTITATION OF PROTEIN CONCENTRATIONS IN PURE OR MIXED SOLUTIONS - ABSTRACT AN ATTRACTIVE FEATURE OF USING IN VITRO EXPRESSION SYSTEMS TO GENERATE BIOLOGICAL COMPOUNDS IS THE CAPABILITY TO PRODUCE A WIDE VARIETY OF COMPOUNDS (PROTEINS, PEPTIDES, ANTIBODIES) ON VARIOUS SCALES, IN A CHEAP AND RAPID MANNER. CURRENT METHODS TO ANALYZE ANY OF THESE PRODUCED COMPOUNDS REQUIRE PURIFICATION WHICH POSES A MAJOR DRAWBACK FOR WORKING WITH PRODUCTS DERIVED FROM EXPRESSION SYSTEMS. FOR EXAMPLE, A MODERATELY ABUNDANT (SOLUBLE) PROTEIN CAN REQUIRE 27 INDIVIDUAL STEPS OVER FOUR DAYS TO PURIFY; A SIGNIFICANT AMOUNT OF TIME AND EXPENSE TO SCREEN A SINGLE PROTEIN. SCREENING A LIBRARY OF POTENTIAL BIOLOGICAL COMPOUNDS FOR THERAPEUTIC VALUE, REQUIRES MANY PURIFICATION STEPS FOR EACH COMPOUND BEFORE ANY ANALYSIS CAN BE PERFORMED. THIS INCLUDES MEASUREMENTS AS SIMPLE AS DETERMINATION OF THE MASS OF THE PRODUCED BIOLOGICAL COMPOUNDS. TO THIS END WE HAVE DEVELOPED A UNIQUE DNA-BASED SCHEME USING DIFFERENTIAL SCANNING CALORIMETRY FOR DETERMINATION OF THE MASSES OF BIOLOGICAL COMPOUNDS IN BOTH PURIFIED AND MIXED MEDIA. UNIQUELY, OUR PROBES ARE UNIVERSALLY APPLICABLE TO A WIDE RANGE OF BIOLOGICAL COMPOUNDS ENABLING MASS QUANTIFICATION PRIOR TO PURIFICATION. THIS ADVANCE ALLOWS FOR INITIAL ANALYSIS OF BIOLOGICS EARLY IN THE PRODUCTION PROCESS, LOWERING ASSOCIATED TIME REQUIREMENTS AND RESOURCE COSTS. AN ADDED BENEFIT IS THE POSSIBILITY TO SUPPLANT THE NUMEROUS MASS ANALYSIS KITS CURRENTLY ON THE MARKET THAT RELY ON COLORIMETRIC OR FLUORESCENT METHODS. THESE METHODS ARE HIGHLY SPECIFIC TO THE TYPES OF COMPOUNDS ASSAYED AND HAVE LIMITATIONS PREVENTING UNIVERSAL APPLICABILITY. OUR PRODUCT OFFERS A SUPERIOR ADVANTAGE OF EXISTING METHODS. IN THIS PROJECT WE PLAN TO SCREEN A LIBRARY OF BIOLOGICALLY EXPRESSED SARS-COV-2 SPIKE AND NUCLEOCAPSID PROTEINS GENERATED BY OUR COMMERCIAL COLLABORATOR. THE AIM OF THIS WORK IS TO EVALUATE WHETHER OUR ASSAY IS CAPABLE OF PREDICTING PROTEIN YIELDS FROM THEIR EXPRESSION SYSTEMS SOLELY FROM A MASS DETERMINATION OF THEIR UNPURIFIED SAMPLES. ADDITIONALLY, WE WILL USE OUR ASSAY TO VERIFY THE PROTEIN STRUCTURE BEFORE AND AFTER PURIFICATION.
Mod # | Description | ReasonForModification | Federal Obligation | Date |
|---|---|---|---|---|
| Not listed | $0 | 6/25/24 | ||
| Not listed | $258.6k | 9/6/22 | ||
| Not listed | $258.6k | 9/6/22 |