Exploring the precise role of heparanase via small molecule probes
Active Project Grant Awarded Last action
- Award ID
- R35GM156219
- Recipient
-
University Of Florida UEI NNFQH1JAPEP3
- Awarding agency
-
National Institute of General Medical Sciences
National Institutes of Health, Department of Health and Human Services
- Obligated
- $864,205
Award facts
- Assistance listing
- 93.859 Biomedical Research and Research Training
- Completion date 31% complete
- Place of performance
- Gainesville, FL 32611, USA
Award details come from USAspending.gov.
Description
- Abstract
- The extracellular matrix (ECM) is an essential component of the microenvironment of cells and is known to provide mechanical supports for organs and tissues, as well as loci for cell adhesion, interaction, and signaling to regulate various cell functions. Heparan sulfate proteoglycans (hspgs), major components in the ECM of all tissue types, maintain the structural integrity of ECM and regulate cellular signaling via binding with ECM components and protein ligands such as growth factors and chemokines. Heparanase, the only known enzyme that can cleave the heparan sulfate (HS) side chains of hspgs and a key enzyme for ECM remodeling, regulates many cellular processes including ECM remodeling and homeostasis of cell-associated HS, and IT controls the bioavailability and activity of molecules attached to HS. The importance of heparanase activity in various pathological conditions such as cancer, angiogenesis, and inflammation has been well demonstrated, however, a detailed mechanistic study of heparanase function and the precise mode of heparanase action in ECM remodeling remain elusive. Molecular probes that can dissect the different functions of heparanase can explore its precise role in ECM remodeling, however, prior to our work, there were no suitable tools for this type of investigation. Our overall research program has been built on the development of novel heparanase probes that have defined structures, and "in situ" labeling chemistry that can facilitate real-time detection of heparanase activity both in vitro and in vivo. With the support of mira-ESI, my lab has successfully developed efficient chemical strategies to yield novel small molecule probes to detect heparanase activity, and "in situ" labeling chemistry suitable for carbohydrate processing enzymes. In the proposed mira application and the long term, we will develop a library of novel heparanase probes with varying glycan substrates and use these structurally defined molecules to study the precise function of heparanase using in vitro and in vivo models involving ECM remodeling.
Transactions
| Action | Obligated | Date |
|---|---|---|
| Amendment | $419,375 | |
| Original award | $444,830 |
Quick answers
Who received award R35GM156219?
R35GM156219 was awarded to University Of Florida by National Institute of General Medical Sciences (National Institutes of Health) on April 11, 2025.
How much is award R35GM156219 worth?
$864,205 has been obligated on R35GM156219.
On GovTribe
See this award's full record
- Every transaction and subaward
- The recipient's other awards and teaming partners
- Similar grant awards
- Ask GovTribe AI about this award