Investigating the molecular regulation of cell protrusion function in 3D
Active Project Grant Awarded Last action
- Award ID
- R35GM155310
- Recipient
-
Regents Of The University Of Minnesota UEI KABJZBBJ4B54
- Awarding agency
-
National Institute of General Medical Sciences
National Institutes of Health, Department of Health and Human Services
- Obligated
- $1,075,477
Award facts
- Assistance listing
- 93.859 Biomedical Research and Research Training
- Completion date 43% complete
- Place of performance
- Minneapolis, MN 55455, USA
Award details come from USAspending.gov.
Description
- Abstract
- Cells shape and reshape themselves as they accomplish diverse functions in vivo. These cell shape changes result from physical and chemical feedback loops at multiple scales. At the biochemical scale, spatiotemporally varying signaling cascades control cytoskeletal polymerization that pushes on the plasma membrane from the inside, inducing cell morphology change at the scale of micrometers. Cell morphology in turn feedbacks to alter intracellular signaling, via mechanisms such as surface curvature-sensing proteins in the plasma membrane. Understanding these feedbacks across scales is particularly critical since they are a target for pharmaceutical intervention. Scientists have developed a wealth of techniques for interrogating the biochemical and genetic basis of cell function. However, disentangling the many feedback loops coupling cell morphology, intracellular organization, and spatiotemporally varying biochemical pathways, such as intracellular signaling, remains EX- perimentally challenging. Live-cell fluorescence microscopy can visualize these feedbacks in action, but imaging across scales produces enormous and detailed datasets that are impossible to make sense of without dedicated computational pipelines. We will develop algorithms for cell biology rooted in computational geometry. Using computational geometry approaches will allow US to draw from decades of math and computer science research to work in biologically relevant geometries, increasing accuracy and easing interpretation. We propose to develop essential algorithms to reconstruct plasma membrane organization from 3D microscopy images and track its movements across time. We will also measure the organizational rules that couple plasma membrane shape and dynamics to spatiotemporally varying biochemical pathways on the cell surface. Finally, we will focus on understanding feedbacks related to intracellular organization throughout the cell, not just on the plasma MEM- brane, by broadening the computational approaches we developed for the plasma membrane to other geome- tries. The computational methods that we are proposing to develop will aid in further opening up to scientific investigation the many feedback loops and physical interactions of the meso-scale world of subcellular organi- zation.
Transactions
| Action | Obligated | Date |
|---|---|---|
| Amendment | $387,500 | |
| Amendment | $387,500 | |
| Original award | $300,477 |
Quick answers
Who received award R35GM155310?
R35GM155310 was awarded to Regents Of The University Of Minnesota by National Institute of General Medical Sciences (National Institutes of Health) on September 1, 2024.
How much is award R35GM155310 worth?
$1,075,477 has been obligated on R35GM155310.
On GovTribe
See this award's full record
- Every transaction and subaward
- The recipient's other awards and teaming partners
- Similar grant awards
- Ask GovTribe AI about this award