Developing single nuclei trna-SEQ to profile trna diversity at the single cell level
Ended Project Grant Awarded Last action
- Award ID
- R21NS139431
- Recipient
-
Oregon Health & Science University UEI NPSNT86JKN51
- Awarding agency
-
National Institute of Neurological Disorders and Stroke
National Institutes of Health, Department of Health and Human Services
- Obligated
- $253,442
Award facts
- Assistance listing
- 93.853 Extramural Research Programs in the Neurosciences and Neurological Disorders
- Completion date
- Place of performance
- Portland, OR 97239, USA
Award details come from USAspending.gov.
Description
- Abstract
- Transfer RNA (trna) biogenesis is essential for determining the pool of trnas that set the translational state of cells, and defects in the trna biogenesis machinery are associated with several types of neurodegenerative diseases. While bulk trna-sequencing assays of tissues and cell cultures have revealed that trna expression and modification are regulated in tissue and disease-specific manners, technology to date has hindered our ability to define trna processing, expression, or modification changes in neurodevelopment and neurodegeneration at the level of single cells, limiting important insight and therapeutic development. In this application, we propose to develop and apply a novel trna sequencing method to stem cell derived neurons and cerebral organoids to reveal complex trna processing and modification patterns at the single cell level for the first time. We will utilize preexisting laboratory models to establish and optimize our technology (aim 1) before profiling cerebral organoids with pathogenic variants in CLP1 and isogenic controls (aim 2). These findings will reveal the complexity of trna processing and modification at unprecedented cellular level resolution. In addition to neurodevelopment and neurodegeneration, our research will have broad impacts on the study of trnas across all tissues, as IT will be applicable to any frozen or fresh tissue source. Outside of neurobiology, IT will also benefit other fields such as cancer biology and virology where trna modulation is known to be important.
Transactions
| Action | Obligated | Date |
|---|---|---|
| Amendment | $161,739 | |
| Amendment | -$27,569 | |
| Amendment | -$161,739 | |
| Amendment | -$161,739 | |
| Original award | $442,750 |
Quick answers
Who received award R21NS139431?
R21NS139431 was awarded to Oregon Health & Science University by National Institute of Neurological Disorders and Stroke (National Institutes of Health) on July 15, 2024.
How much is award R21NS139431 worth?
$253,442 has been obligated on R21NS139431.
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