The National Human Genome Research Institute (NHGRI), through the Human Genome Research program (CFDA 93.172), awarded a $444,125 Project Grant to the Rector & Visitors of the University of Virginia (UVA) to develop computational models that accurately quantify open chromatin bias and TN5 intrinsic cleavage bias in both bulk and single-cell Cleavage Under Targets & Tagmentation (CUT & TAG) data. The models will characterize how these biases affect the detection of histone modifications and transcription factor binding sites, and integrate the bias correction into existing or new bioinformatics methods for improved CUT & TAG data analysis. This work aims to fill knowledge gaps in single-cell studies of chromatin dynamics and transcriptional regulation, with potential applications in functional genomics and epigenomics research. The project will be conducted by UVA over a 2-year period from September 2023 to August 2025.
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