Project Grant R21DA063110

Award Date 4/15/25
Completion Date 3/31/27
Dollars Obligated $234K
Federal Grant Program
93.279
Assistance Type
Project Grant
Place of Performance
Washington, DC, USA
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This Project Grant award, with a total funding of $234,000.00, was provided by the National Institute on Drug Abuse (NIDA) under the Drug Use and Addiction Research Programs (CFDA 93.279). The grant aims to understand the effect of opioids on HIV infection and the reactivation of latent viral reservoirs in the brain.

Specifically, the grant will utilize a novel dual fluorescent and bioluminescent HIV virus, called EcoHIV NL4-3 Dual Red, to visualize and measure the impact of opioids, including fentanyl, morphine, and methadone, on HIV proviral transcription in microglia and astrocytes (Aim 1). Additionally, the grant will employ latent HIV infection in rats and in vivo imaging systems to longitudinally assess viral reactivation in the brain during opioid administration (Aim 2). The results from this project are expected to identify key pathways involved in opioid-mediated activation of HIV in glial cells and establish a timeline of viral reactivation, which could inform treatment strategies for people living with HIV who use opioids.

The grant was awarded to Georgetown University, a private, non-profit research institution, and is set to run from April 15, 2025, to March 31, 2027.

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