Project Grant R21AG093151

Award Date 6/1/25
Completion Date 5/31/27
Dollars Obligated $461K
Federal Grant Program
93.866
Assistance Type
Project Grant
Place of Performance
New Haven, CT 06510, USA
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This federal Project Grant award from the National Institute on Aging (CFDA 93.866 - Aging Research) provides $656,709 to Yale University to define the molecular and ultrastructural mechanisms that underpin the process of nucleophagy - the lysosome-mediated degradation of nuclear components. The 5-year research project, beginning December 1, 2024, aims to leverage the yeast model system to characterize the timeline and molecular machineries involved in clearing dysfunctional nuclear proteins,...
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The key objectives are to characterize synaptic vesicles, synaptic vesicles targeted for autophagy, and synaptic autophagosomes in wildtype and CSPalpha knockout models using mass spectrometry and electron microscopy. The researchers will also investigate the relationship between the synaptic vesicle cycle and autophagosome biogenesis by tagging synaptic vesicle proteins with an autophagy reporter in wildtype, CSPalpha knockout, and Cln4 (neurodegeneration) cell culture models. This research aims to advance the understanding of presynaptic autophagy, synaptic vesicle turnover, and their links to neurodegenerative diseases like Cln4 and age-related dementias.

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