The integration of laboratory data with computational 3-d modeling to analyze the role of the central amygdala in neuropathic pain
Active Project Grant Awarded Last action
- Award ID
- R15NS128624
- Recipient
-
Duquesne University Of The Holy Spirit UEI NGYSJ2L1LZX3
- Awarding agency
-
National Institute of Neurological Disorders and Stroke
National Institutes of Health, Department of Health and Human Services
- Obligated
- $441,500
Award facts
- Assistance listing
- 93.853 Extramural Research Programs in the Neurosciences and Neurological Disorders
- Completion date 79% complete
- Originally
- Place of performance
- Pittsburgh, PA 15219, USA
Award details come from USAspending.gov.
Description
- Abstract
- Neuropathic pain impacts the lives of millions of individuals worldwide. Understanding the mechanisms that drive the development and maintenance of neuropathic pain is critical to the advancement of next generation therapeutic strategies. In the context of nerve injury, one brain area that may contribute to pain is the central nucleus of the amygdala (CEA). Evidence from human patients and animal models shows short and long-term changes in the amygdala may contribute to the overall pathologic state. An explosion of cell-type specific optogenetic, chemogenetic, and physiological approaches has provided unprecedented cellular access to the CEA in the context of injury. A major challenge for the field is determining how to integrate data from different approaches and laboratories to understand the amygdala's contribution to nociception and pain. We recently developed the first computational model of the CEA in the context of pain using real-world cell-type data. This model, built with physiological data and validated against in vivo results, provides a robust framework to study the interactions between different cell types and their collective contributions to the development of pain. Our model of the CEA marks a major step forward in the study of neuropathic pain, but at the same time the model is still primitive in its assumptions. The model represents the CEA in 2-dimensional space and fails to accurately capture the complex 3-dimensional (3-d) structural properties of the CEA and its subnuclei that likely drive pronociceptive and antinociceptive outputs from the CEA. The objective of this proposal is to utilize existing and new wet-lab data to build a proof-of-concept 3-d model of pain-related neurons in the CEA that accounts for spatial and cell-type heterogeneity and determine the extent to which the model can predict in vivo results. Our central hypothesis is that the 3-d distribution of two specific cell types in the CEA influences the appropriate behavioral responses in the context of neuropathic injury and that this distribution can be modeled to produce novel insights into the CEA's role in the development of chronic nociception. We will use an agent-based computational model to describe the physiological and morphological properties of individual neurons in the CEA and their interactions with one another. This approach will allow US to capture complexity within the CEA system while remaining accessible to undergraduate students, including those new to programming. The computational model, which will be publicly available and open source, will include a graphical user interface that can be accessed by any researcher to complete in silico experiments prior to devoting the time and resources to costly in vivo experiments. Both computational and wet-lab experiments are incorporated in this proposal with a focus on involvement of undergraduate students to bolster their development as rigorous, interdisciplinary, and thoughtful biomedical researchers.
Transactions
| Action | Obligated | Date |
|---|---|---|
| Amendment | $0 | |
| Original award | $441,500 |
Subawards
| Subrecipient | Amount | Awarded |
|---|---|---|
| University Of Texas At Dallas | $156,000 |
Quick answers
Who received award R15NS128624?
R15NS128624 was awarded to Duquesne University Of The Holy Spirit by National Institute of Neurological Disorders and Stroke (National Institutes of Health) on September 7, 2023.
How much is award R15NS128624 worth?
$441,500 has been obligated on R15NS128624.
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