Regulation of cardiac patterning via akirin/nurd interactions
Active Project Grant Awarded Last action
- Award ID
- R15HL161738
- Recipient
-
Kennesaw State University UEI V1WUYJCYJVJ4 Part of Board Of Regents Of The University System Of Georgia
- Awarding agency
-
National Heart Lung and Blood Institute
National Institutes of Health, Department of Health and Human Services
- Obligated
- $812,534
Award facts
- Assistance listing
- 93.837 Cardiovascular Diseases Research
- Completion date 85% complete
- Originally
- Place of performance
- Kennesaw, GA 30144, USA
Award details come from USAspending.gov.
Description
- Abstract
- Congenital heart defects are the most prevalent birth defects in the human population, with an incident rate as high as 10 in 1000 live births. A signicant number of these cases are termed sporadic, which are largely the result of interactions between a number of independent genetic loci and alleles. To aid in our understanding of the polygenic nature of congenital heart defects, IT remains imperative to continue to identify new gene regulatory partners that may play a role in the process of embryonic heart patterning. Our laboratory has recently identied a cofactor, akirin, that is responsible for interfacing transcription factor activity with chromatin remodeling machinery to facilitate gene expression. Importantly, akirin appears to play a role in the proper patterning and morphogenesis of the embryonic heart in drosophila melanogaster. Excitingly, our preliminary data suggests that akirin likely regulates insect embryonic heart development through interactions with the CHD4/nurd family of chromatin remodeling machinery. This mechanism of akirin/nurd interactions for expression of cardiac developmental gene pathways appears to be conserved from mammals to insects. To further determine the role of akirin/nurd interactions in the process of heart development, we propose two specic aims: 1) using a combination of genetic, biochemical, and live imaging techniques, will conrm the importance of akirin/nurd interactions during development, and 2) we will employ both next-generation massively parallel RNA sequencing methods, as well as atac-SEQ and conventional chromatin immunoprecipitation methods to determine the gene regulatory role of akirin during cardiac myogenesis. This work will provide key data for understanding the role of akirin in the process of heart formation, and provide a new avenue for studying and/or preventing causes of congenital heart defects. Critically, in keeping with the goals of the area award mechanism, this project will give undergraduate researchers hands-on experience in a wide variety of molecular, genetic, histological, microscopic, and biochemical techniques, which will provide a valuable skill set for a future career in biomedical research.
Transactions
| Action | Obligated | Date |
|---|---|---|
| Amendment | $0 | |
| Amendment | $0 | |
| Amendment | $0 | |
| Amendment | $406,267 | |
| Original award | $406,267 |
Quick answers
Who received award R15HL161738?
R15HL161738 was awarded to Kennesaw State University by National Heart Lung and Blood Institute (National Institutes of Health) on September 5, 2022.
How much is award R15HL161738 worth?
$812,534 has been obligated on R15HL161738.
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