Cell plasticity in the origin of gastric carcinogenesis

Ended Project Grant Awarded Last action

Award ID
R01CA272687
Recipient
Awarding agency
Obligated
$9,365,903

Award facts

Assistance listing
93.396 Cancer Biology Research
Completion date
Place of performance
Nashville, TN 37203, USA

Award details come from USAspending.gov.

Description

As the agency reported it.

Abstract
Gastric cancer is the 4TH leading cause of cancer-related death worldwide and IT most commonly develops within a carcinogenic cascade from pre-cancerous metaplasia to dysplasia and adenocarcinoma. Metaplasias first arise as a response to injury through the chief cell transdifferentiation into spasmolytic polypeptide- expressing metaplasia (spem) cells. While this initial process is possibly reversible, oncogenic gene activation or chronic inflammation can activate spem cell plasticity, which promotes spem cell progression to intestinal metaplasia (IM) and dysplasia. This neoplastic process may also lead to transcriptional and epigenetic changes, and incite cell lineage conversion, where multiple intermediate cell types are produced that can evolve into cancerous cells, including dysplastic stem cells which may arise during the neoplastic transition. Furthermore, the oncogenic gene mutation burden may be associated with the cell lineage conversion and diversification of the dysplastic stem cells to cancerous cells. However, IT is not clear whether the spem cell plasticity is responsible for the cell heterogeneity and evolution of pre-cancerous metaplasia to incomplete IM, which carries a higher risk of patient progression to dysplasia and what mechanisms are involved in the carcinogenic process. We therefore hypothesize that spem cells are key gastric cancer precursor cells, which display functional properties and cell lineage conversion capacity to drive metaplasia progression to dysplasia. Our overarching goal is to define mechanisms that control the cell lineage conversion of reparative spem cells towards incomplete IM and more cancerous cell lineages, which display a higher mutational burden. To address these questions directly, we have established novel in vivo transgenic mouse models and in vitro metaplastic or dysplastic organoid models derived from transgenic mouse stomachs following induction of active kras or from human patient samples with metaplasia or dysplasia. Using these novel models, we will assess critical spem cell lineage derivation and define cell populations that account for the key transcriptional and epigenetic changes arising during metaplasia progression. We will pursue three specific aims: first we will assess functional properties of spem cells during mucosal recovery or neoplastic progression following mucosal injury. Second, we will examine regulatory mechanisms of cell lineage diversification and conversion during metaplasia progression. Third, we will investigate molecular mechanisms driving cell linage diversification and clonal evolution of dysplastic stem cells to adenocarcinoma. Our studies will define critical transition points which lead to neoplastic transitions for spem cells as the origin of gastric carcinogenesis. An understanding of regulatory mechanisms in cell plasticity and the ability to reverse such transitions could lead to therapeutic interventions to prevent gastric cancer.

Transactions

Action Obligated Date
Amendment $0
Amendment $2,053,139
Amendment $2,053,139
Amendment $0
Amendment $0
Amendment $975,011
Amendment Hierarchy of oncogenic gene mutations in gastric carcinogenesis $184,000
Amendment $1,013,248
Amendment Hierarchy of oncogenic gene mutations in gastric carcinogenesis $1,013,248
Amendment $1,037,059
Original award $1,037,059
Show all 11

Subawards

Subrecipient Amount Awarded
Vanderbilt University Cell plasticity in the origin of gastric carcinogenesis $13,650
Vanderbilt University $20,933
Vanderbilt University $14,857

Quick answers

Who received award R01CA272687?

R01CA272687 was awarded to Vanderbilt University Medical Center by National Cancer Institute (National Institutes of Health) on September 1, 2022.

How much is award R01CA272687 worth?

$9,365,903 has been obligated on R01CA272687.

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