Project Grant K99AI194970

Award Date 7/7/25
Completion Date 6/30/27
Dollars Obligated $132K
Federal Grant Program
93.855
Assistance Type
Project Grant
Place of Performance
Ann Arbor, MI 48109, USA
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This Project Grant award from the National Institute of Allergy and Infectious Diseases (NIAID), under the Allergy and Infectious Diseases Research program (CFDA 93.855), provides $132,300.00 to the Regents of the University of Michigan to conduct research on the multifaceted roles of phosphatidylinositol (4,5)-bisphosphate [PI(4,5)P2] in HIV-1 assembly. The key research objectives are to:

  1. Elucidate the interplay between Gag multimerization and PI(4,5)P2 enrichment at HIV-1 assembly sites, and how this facilitates the sorting of cellular proteins that enhance or inhibit HIV-1 spread.

  2. Investigate the role of PI(4,5)P2 in the recruitment and incorporation of the HIV-1 glycoprotein Env into viral particles across cell lines and primary CD4+ T cells.

  3. During the independent research phase (R00), focus on determining whether Gag is addressed to pre-formed PI(4,5)P2 pools at the plasma membrane and the role of membrane contact sites in HIV-1 protein trafficking and assembly.

The award period is from July 7, 2025 to June 30, 2027. This research aims to address significant gaps in the understanding of HIV-1 assembly mechanisms, which can contribute to the development of novel therapeutic approaches.

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