Project Grant R01AI192025

Award Date 4/24/25
Completion Date 3/31/30
Dollars Obligated $860K
Federal Grant Program
93.855
Assistance Type
Project Grant
Place of Performance
Hamden, CT 06511, USA
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This Project Grant award from the National Institute of Allergy and Infectious Diseases (NIAID), under the Allergy and Infectious Diseases Research program (CFDA 93.855), supports molecular investigations of HIV-1 maturation pathways and the actions of inhibitor compounds within native cellular environments. The $859,992 award, effective from Apr 24, 2025 to Mar 31, 2030, will enable the Xiong Laboratory at Yale University to utilize advanced in situ cryo-electron microscopy techniques to:

  1. Determine the high-resolution structural pathway of HIV-1 Gag assemblies and capsid during maturation within their native environment, including both purified virus-like particles and budding viruses from infected cells.

  2. Define the mechanisms by which HIV-1 capsid cofactors and maturation inhibitors, such as lenacapavir, influence the virus maturation process during budding.

The insights gained from this research are expected to advance the understanding of HIV-1 maturation, guide the development of new therapeutic strategies targeting the immature Gag structures and capsid, and provide valuable tools for studying host-virus interactions within their native cellular environments.

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