This Project Grant award from the National Institute of Allergy and Infectious Diseases (NIAID), under the Allergy and Infectious Diseases Research program (CFDA 93.855), supports molecular investigations of HIV-1 maturation pathways and the actions of inhibitor compounds within native cellular environments. The $859,992 award, effective from Apr 24, 2025 to Mar 31, 2030, will enable the Xiong Laboratory at Yale University to utilize advanced in situ cryo-electron microscopy techniques to: Determine the high-resolution structural pathway of HIV-1 Gag assemblies and capsid during maturation within their native environment, including both purified virus-like particles and budding viruses from infected cells. Define the mechanisms by which HIV-1 capsid cofactors and maturation inhibitors, such as lenacapavir, influence the virus maturation process during budding. The insights gained from this research are expected to advance the understanding of HIV-1 maturation, guide the development of new therapeutic strategies targeting the immature Gag structures and capsid, and provide valuable tools for studying host-virus interactions within their native cellular environments.
Mod # | Description | Reason For Modification | Federal Obligation (Click to sort descending) | Date (Click to sort ascending) |
|---|---|---|---|---|
| Not listed | $860.0k | 4/24/25 |