A dynamic and three-dimensional human model of hypoplastic left heart syndrome to probe compromised cardiomyocyte proliferation
Active Project Grant Awarded Last action
- Award ID
- F32HL170581
- Recipient
-
Research Institute At Nationwide Children's Hospital UEI EYMJXLN2MFB4
- Awarding agency
-
National Heart Lung and Blood Institute
National Institutes of Health, Department of Health and Human Services
- Obligated
- $312,920
Award facts
- Assistance listing
- 93.837 Cardiovascular Diseases Research
- Completion date 70% complete
- Place of performance
- Columbus, OH 43205, USA
Award details come from USAspending.gov.
Description
- Abstract
- Hypoplastic left heart syndrome (hlhs) is a severe congenital heart defect in which the left side of the heart is underdeveloped, with structural malformations to the ascending aorta, aortic/mitral valves, and a characteristically small left ventricle. These anatomical abnormalities restrict proper blood circulation in infants and can be lethal if left untreated. While palliative procedures have improved the prognosis of hlhs patients, many still suffer downstream morbidities and a diminished quality of life. Unfortunately, the etiology of hlhs remains elusive to scientists and clinicians. Genetic and environmental factors are thought to contribute to the development of the disease; however, the exact cellular processes that modulate hlhs pathogenesis still need to be elucidated. An inadequate understanding of the syndrome is exacerbated by limited experimental models that can recapitulate and manipulate key features of hlhs. Restricted ethical access to human embryos, and fundamental differences between animals and humans have failed to produce models that mimic clinical phenotypes. Furthermore, standard 2D in-vitro cell culture spatially restricts tissue growth, which does not fully recap three-dimensional development in nature. When compared to 2D culture, cardiomyocytes grown in 3D have shown enhanced sarcomeric structure, contractility, mitochondrial respiration, cellular alignment, and electrophysiological characteristics. Therefore, there exists a need to develop alternative strategies to study abnormal cardiac development in-vitro. Tissue engineering techniques such as 3D bioprinting offer a unique platform to deposit cells and biomaterials that mimic the extracellular matrix of tissues in an architecturally controlled fashion. In addition to geometric control, the properties of these cell-laden hydrogels, such as stiffness, can be controlled. Moreover, advances in ipsc technology offer an in-vitro biological system that retains genetic- and disease-specific information from donors which provides a tool to probe genetically inheritable and developmental diseases. Leveraging these technological advances, our proposed study aims to utilize a 3D- bioprinted heart tube patterned into an endocardial layer (with ipsc-derived endothelial cells) and myocardial layer (with ipsc-derived cardiomyocytes) to interrogate how dysregulated fluid-induced biomechanics and microenvironmental stiffness impedes cardiac proliferation in-vitro. In this regard, we hypothesize aberrant biomechanical forces induce stress-related endocardial-myocardial signaling that ultimately impedes cardiomyocyte proliferation. We will test our hypothesis by (1) selectively varying endocardial stiffness within our disease-specific 3D model and assessing intercellular signaling that dysregulates cardiomyocyte proliferation, and (2) by applying varying degrees of flow-induced shear stress to the endocardial layer and studying transcriptional shifts that occur in response to these perturbations at the single-cell level. Successful completion of these aims will give novel etiological insights into how dysregulated biomechanical cues in hlhs impedes cardiac growth and could shed light into alternative approaches to treat ventricular hypoplasia.
Transactions
| Action | Obligated | Date |
|---|---|---|
| Amendment | $84,148 | |
| Amendment | $80,224 | |
| Amendment | $4,964 | |
| Amendment | $0 | |
| Amendment | $71,792 | |
| Original award | $71,792 |
Quick answers
Who received award F32HL170581?
F32HL170581 was awarded to Research Institute At Nationwide Children's Hospital by National Heart Lung and Blood Institute (National Institutes of Health) on September 30, 2024.
How much is award F32HL170581 worth?
$312,920 has been obligated on F32HL170581.
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