Investigating the functional role of a degron in ASXL1 in hematopoiesis and disease
Ended Project Grant Awarded Last action
- Award ID
- F31HL174076
- Recipient
-
President And Fellows Of Harvard College UEI LN53LCFJFL45
- Awarding agency
-
National Heart Lung and Blood Institute
National Institutes of Health, Department of Health and Human Services
- Obligated
- $233,852
Award facts
- Assistance listing
- 93.837 Cardiovascular Diseases Research
- Completion date
- Place of performance
- Cambridge, MA 02138, USA
Award details come from USAspending.gov.
Description
- Abstract
- ASXL1 is one of the most mutated genes in hematological disorders. ASXL1 mutations occur in all types of myeloid malignancies, including 45% of chronic myelomonocytic leukemia cases where they are associated with poor prognosis; and ASXL1 is the third-most mutated gene in clonal hematopoiesis, a condition defined by aberrant expansion of hematopoietic stem cells (hscs), which increases the risk of cardiovascular disease and other hematological disorders. Despite the prevalence and significance of ASXL1 mutations, IT is poorly understood how they are mechanistically linked with these hematological disorders as very little is known about the protein itself. ASXL1 is a scaffolding component of the polycomb-repressive deubiquitinase (PR-DUB) complex, which antagonizes polycomb-mediated repression to regulate critical gene expression programs. Many ASXL1 mutations are frameshift or nonsense mutations in the final exon that result in truncated protein products. While early work focused on the effects of knocking out ASXL1, recent work has shown that truncated ASXL1 is actually present at higher levels than the wild-type protein. This stabilizes and hyperactivates PR-DUB, ultimately leading to epigenetic dysregulation. However, the molecular basis of how ASXL1 truncation increases its abundance and whether this drives its disease function remain unclear. In my preliminary studies, i discovered a degron in the last exon of ASXL1 that is necessary and sufficient for its degradation by the ubiquitin proteasome system. This proposal will test my hypothesis that the highly recurrent ASXL1-truncating mutations remove this degron to aberrantly stabilize the protein, leading to formation of hyperactive PR-DUB complexes, DE-repression of polycomb-target genes, and HSC expansion. To test this hypothesis, i will use a fluorescence-based cellular reporter of protein stability to further characterize this degron and perform a crispr screen to identify the E3 ubiquitin ligase that recognizes the degron. Then i will establish the relevance of this mechanism to the clonal expansion phenotype in primary hscs by crispr mutagenesis paired with single-cell RNA sequencing (i.e., perturb-SEQ). In the long term, this knowledge will advance our fundamental understanding of ASXL1's role in primary hematopoiesis, how clinical mutations disrupt that process, and propel the development of new therapeutic strategies for a wide array of hematological disorders.
Transactions
| Action | Obligated | Date |
|---|---|---|
| Amendment | $564 | |
| Amendment | $35,118 | |
| Amendment | $35,118 | |
| Amendment | $1,280 | |
| Amendment | $1,280 | |
| Amendment | $40,123 | |
| Amendment | $0 | |
| Amendment | $40,123 | |
| Amendment | $40,123 | |
| Amendment | $0 | |
| Amendment | $0 | |
| Original award | $40,123 |
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Quick answers
Who received award F31HL174076?
F31HL174076 was awarded to President And Fellows Of Harvard College by National Heart Lung and Blood Institute (National Institutes of Health) on September 4, 2024.
How much is award F31HL174076 worth?
$233,852 has been obligated on F31HL174076.
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