Investigating the contributions of chondrocyte spindle biophysics and tissue microenvironment to skeletal dysplasia
Active Project Grant Awarded Last action
- Award ID
- F31AR087752
- Recipient
-
University Of Vermont & State Agricultural College UEI Z94KLERAG5V9
- Awarding agency
-
National Insititue of Arthritis and Musculoskeletal and Skin Diseases
National Institutes of Health, Department of Health and Human Services
- Obligated
- $70,404
Award facts
- Assistance listing
- 93.846 Arthritis, Musculoskeletal and Skin Diseases Research
- Completion date 0% complete
- Originally
- Place of performance
- Burlington, VT 05405, USA
Award details come from USAspending.gov.
Description
- Abstract
- Our understanding of how the cell division process varies across different tissues at the molecular level is extremely limited. Most mitotic proteins are ubiquitously expressed, but dysregulation of these highly coordinated component parts can still lead to dysfunction in a tissue-specific manner. One such example can be found in patients with spondyloepimetaphyseal dysplasia with joint laxity type 2 (SEMDJL2), who present with skeletal tissue-specific defects in development. SEMDJL2-causing point mutations have been identified in the ubiquitous mitotic chromokinesin KIF22. We have shown that SEMDJL2-associated mutations in KIF22 disrupt chromosome segregation in anaphase and result in reduced proliferation, abnormal daughter cell nuclear morphology, and cytokinesis failure. Interestingly, the mutations did not disrupt KIF22 motor function or localization, but rather the mechanisms that regulate KIF22 activity over the course of mitosis, leaving the motor hyperactivated. Persistent KIF22 motor activity during anaphase impairs the separation of chromosomes after alignment, which subsequently results in cytokinesis defects. However, IT remains to be determined how these effects cause skeletal tissue-specific defects, since KIF22 is ubiquitously expressed. Spatial constraints in the skeletal growth plate may restrain spindle pole separation in anaphase and impair cytokinesis by preventing daughter cells from pulling away from one another during division. These impairments may be amplified by aberrant polar ejection forces to a point where the cell can no longer divide normally. We predict that the skeletal tissue-specific sensitivity to activating mutations in KIF22 is due to a combination of chondrocyte-specific characteristics that impact the force balance within mitotic spindles and the spatial constraints imposed by the skeletal growth plate. Investigating the molecular mechanisms by which mutations in KIF22 selectively disrupt growth of skeletal tissue in SEMDJL2 patients will additionally provide insight into the chondrocyte-specific functional roles of KIF22, the regulatory mechanisms governing the activity of KIF22 during mitosis, and the effects of tissue architecture on cell division.
Transactions
| Action | Obligated | Date |
|---|---|---|
| Amendment | $0 | |
| Amendment | $35,202 | |
| Original award | $35,202 |
Quick answers
Who received award F31AR087752?
F31AR087752 was awarded to University Of Vermont & State Agricultural College by National Insititue of Arthritis and Musculoskeletal and Skin Diseases (National Institutes of Health) on November 16, 2026.
How much is award F31AR087752 worth?
$70,404 has been obligated on F31AR087752.
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