Studies on the sars-COV-2 main protease dimerization mechanism
Ended Project Grant Awarded Last action
- Award ID
- F31AI189116
- Recipient
-
The University Of Texas Health Science Center At San Antonio UEI C3KXNLTAAY98
- Awarding agency
-
National Institute of Allergy and Infectious Diseases
National Institutes of Health, Department of Health and Human Services
- Obligated
- $116,022
Award facts
- Assistance listing
- 93.855 Allergy and Infectious Diseases Research
- Completion date
- Place of performance
- Texas, USA
Award details come from USAspending.gov.
Description
- Abstract
- The main protease enzyme (mpro) of sars-COV-2 cleaves the viral polyprotein into functional units responsible for virus replication and pathogenesis. Interestingly, mpro functions only as a homodimer. However, the structure- function relationship and conformational plasticity of the mpro dimerization mechanism remains poorly understood. To address these gaps in knowledge, i have developed quantitative luciferase-based reporter assays for mpro dimerization in living cells and in vitro. I propose first to use these assays to study the mpro dimerization mechanism through the construction and analysis of a panel structure-guided and evolution- informed mutants, as well as through targeted deep-mutational scanning of dimerization interface residues. Second, i will use my luciferase-based reporter assays to identify candidate dimerization inhibitors. Third, i additionally propose to use my luciferase-based reporter assays to perform studies on the (likely allosteric) mechanism of inhibitor-facilitated dimerization. Altogether, the proposed studies will yield new assays for studying coronavirus protease biology, unique insights into the mechanisms of mpro dimerization and its allosteric modulation, and novel compounds that can be used as chemical probes of these molecular mechanisms.
Transactions
| Action | Obligated | Date |
|---|---|---|
| Amendment | $0 | |
| Amendment | $38,674 | |
| Amendment | $38,674 | |
| Amendment | $0 | |
| Amendment | $0 | |
| Original award | $38,674 |
Quick answers
Who received award F31AI189116?
F31AI189116 was awarded to The University Of Texas Health Science Center At San Antonio by National Institute of Allergy and Infectious Diseases (National Institutes of Health) on May 20, 2025.
How much is award F31AI189116 worth?
$116,022 has been obligated on F31AI189116.
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