Regulation of microbial trafficking to the thymus in early life
Active Project Grant Awarded Last action
- Award ID
- F31AI183752
- Recipient
-
Weill Medical College Of Cornell University UEI YNT8TCJH8FQ8
- Awarding agency
-
National Institute of Allergy and Infectious Diseases
National Institutes of Health, Department of Health and Human Services
- Obligated
- $341,962
Award facts
- Assistance listing
- 93.855 Allergy and Infectious Diseases Research
- Completion date 81% complete
- Place of performance
- New York, NY 10065, USA
Award details come from USAspending.gov.
Description
- Abstract
- Early life is a critical period for the proper development of the immune system. As mammalian hosts have CO- evolved with their symbiotic bacteria, signals from the commensal microbiota are crucial for the prevention of allergy, inflammatory disorders, and protection against enteric pathogens. Our lab has recently uncovered that commensal antigens are trafficked to the thymus by dendritic cells, where they are presented to developing thymocytes. Rather than inducing deletion or treg development, this results in an expansion of naïve microbiota- specific t cell populations, which are functional and egress to populate the peripheral tissue. This process is limited to early life, suggesting that IT is critical for the establishment of the commensal-reactive t cell repertoire. Analysis of thymic 16S sequencing from mice at weaning reveals a stark enrichment of select taxa, such as members of escherichia. When considering the vast diversity of the intestinal microbiome, these data imply an antigenic filter through which only select microbes are trafficked to the thymus. This proposal seeks to understand how the unique environment of the early-life intestine promotes commensal ferrying to the thymus, and the microbial and host factors that curate specific taxa for sampling by sub-epithelial dendritic cells. My central hypothesis is that this process has evolved to prioritize the generation of naïve t cell clones capable of recognizing antigens associated with epithelial invasion. We posit that the signals promoting commensal antigen trafficking to the thymus are 1) adherence to the intestinal epithelium and 2) opsonization by maternal IGG. As recent publications have identified commensal-specific IGG as targeting microbes with the ability to translocate, both of these signals indicate the capacity for epithelial invasion - whether defined by the neonate or by maternal cues. Preliminary data indicate that non-adherent commensals are significantly underrepresented in the thymic tissue. I have also found increased expression of the IGG receptor fcrn in migratory thymic DCS in early life, indicating their enhanced ability to recognize and process IGG-opsonized commensals. In aim 1, i will quantify the epithelial adherence of a model commensal e. Coli throughout early development. I will genetically target adhesins in this commensal to determine how these factors impact epithelial adherence and thymic trafficking in vivo. I will utilize 16S sequencing to compare the mucosa-associated microbiome with that of the thymus. Aim 2 will focus on the role of maternal IGG opsonization, and will quantify thymic trafficking in b cell- and antibody- deficient mouse models throughout early life development. I will manipulate maternal IGG titers through transfer of commensal-specific IGG into IGG-deficient dams, and the impacts on thymic trafficking in offspring will be examined. The proposed experiments will reveal crucial elements of how the commensal-reactive t cell repertoire is curated in early life, and will have important insight into how the microbiome affects the development of the mucosal immune system.
Transactions
| Action | Obligated | Date |
|---|---|---|
| Amendment | $50,114 | |
| Amendment | $564 | |
| Amendment | $48,974 | |
| Amendment | $48,974 | |
| Amendment | $1,280 | |
| Amendment | $1,280 | |
| Amendment | $47,694 | |
| Amendment | $0 | |
| Amendment | $47,694 | |
| Amendment | $47,694 | |
| Amendment | $0 | |
| Amendment | $47,694 | |
| Original award | $0 |
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Quick answers
Who received award F31AI183752?
F31AI183752 was awarded to Weill Medical College Of Cornell University by National Institute of Allergy and Infectious Diseases (National Institutes of Health) on August 20, 2024.
How much is award F31AI183752 worth?
$341,962 has been obligated on F31AI183752.
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