Elucidating the molecular function of kaposi's sarcoma-associated herpesvirus packaging accessory protein ORF68
Active Project Grant Awarded Last action
- Award ID
- F31AI181429
- Recipient
-
Yale Univ UEI FL6GV84CKN57
- Awarding agency
-
National Institute of Allergy and Infectious Diseases
National Institutes of Health, Department of Health and Human Services
- Obligated
- $102,534
Award facts
- Assistance listing
- 93.855 Allergy and Infectious Diseases Research
- Completion date 68% complete
- Place of performance
- New Haven, CT 06511, USA
Award details come from USAspending.gov.
Description
- Abstract
- The standard treatments against other human herpesviruses have limited efficacy against kaposi's sarcoma- associated herpesvirus (kshv). A mechanistic understanding of kshv infection and virion production will help US develop new therapies to limit spread of the virus and thereby prevent the associated diseases, such as kaposi's sarcoma. Viral packaging is an essential, conserved process in herpesviruses and is therefore a promising therapeutic target. Although the essential proteins and necessary molecular events of packaging are known, we lack a detailed mechanistic understanding of the process. These knowledge gaps must be addressed to enable rational drug design. ORF68 is an essential packaging protein of unknown function whose role in this multi-step packaging process is unknown. Recombinant ORF68 forms a pentamer with a positively central channel that binds dsdna in vitro. This work will bridge a critical knowledge GAP between these in vitro observations and ORF68 molecular function during infection in vivo. In aim 1, i will determine the oligomeric state of ORF68 during kshv infection in vivo. In aim 2, i will determine if the ORF68 oligomerization interface is required during kshv infection in vivo. In aim 3, i will directly assess whether DNA binds inside the ORF68 pentamer channel. This work will unveil the molecular function of ORF68 in the context of infection, bringing US closer to a mechanistic model of kshv packaging.
Transactions
| Action | Obligated | Date |
|---|---|---|
| Amendment | $34,574 | |
| Amendment | $33,980 | |
| Amendment | $0 | |
| Original award | $33,980 |
Quick answers
Who received award F31AI181429?
F31AI181429 was awarded to Yale Univ by National Institute of Allergy and Infectious Diseases (National Institutes of Health) on January 19, 2025.
How much is award F31AI181429 worth?
$102,534 has been obligated on F31AI181429.
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