Single-cell multi-omic analysis of cell differentiation in HIV infection
Ended Project Grant Awarded Last action
- Award ID
- F31AI177258
- Recipient
-
Regents Of The University Of Michigan UEI GNJ7BBP73WE9
- Awarding agency
-
National Institute of Allergy and Infectious Diseases
National Institutes of Health, Department of Health and Human Services
- Obligated
- $252,986
Award facts
- Assistance listing
- 93.855 Allergy and Infectious Diseases Research
- Completion date
- Place of performance
- Michigan, USA
Award details come from USAspending.gov.
Description
- Abstract
- Single-cell multi-omic datasets, in which multiple molecular modalities are profiled within the same cell, provide a unique opportunity to discover the temporal relationship between epigenome and transcriptome. To realize this potential, we propose to develop a system consisting of differential equations that extends the RNA velocity framework for gene expression to incorporate epigenomic data. By fitting pairs of jointly sequenced RNA-SEQ and atac-SEQ data, this probabilistic latent variable model is able to estimate the switch time and rate parameters of chromatin accessibility and gene expression from single-cell data, providing a quantitative summary of the temporal relationship between epigenomic and transcriptomic changes. The parameters inferred by the method quantify the length of time for which genes occupy each of the four distinct and biological meaningful states, ranking genes by the degree of coupling between transcriptome and epigenome. Similar to how transcriptomic data has been split into unspliced and spliced modalities to compute RNA velocity, the chromatin accessibility data measured with atac-SEQ can be further split into enhancers and promoters to model dynamics inside CIS-regulatory networks during transcription. We will use this comprehensive multi-layer velocity method to study human blood cell differentiation and mechanistic changes during transcription due to HIV infection. We seek to uncover previously unknown markers of infection status through the means of mathematical modeling on single-cell multi-omic sequencing techniques.
Transactions
| Action | Obligated | Date |
|---|---|---|
| Amendment | $564 | |
| Amendment | $42,809 | |
| Amendment | $42,809 | |
| Amendment | $1,280 | |
| Amendment | $1,280 | |
| Amendment | $41,061 | |
| Amendment | $41,061 | |
| Amendment | $0 | |
| Amendment | $0 | |
| Amendment | $41,061 | |
| Amendment | $0 | |
| Original award | $41,061 |
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Quick answers
Who received award F31AI177258?
F31AI177258 was awarded to Regents Of The University Of Michigan by National Institute of Allergy and Infectious Diseases (National Institutes of Health) on June 1, 2024.
How much is award F31AI177258 worth?
$252,986 has been obligated on F31AI177258.
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