Investigating the mechanism of infection and therapeutic potential of pseudomonas nucleus-forming phages
Active Project Grant Awarded Last action
- Award ID
- F30AI176696
- Recipient
-
University Of California, San Diego UEI UYTTZT6G9DT1
- Awarding agency
-
National Institute of Allergy and Infectious Diseases
National Institutes of Health, Department of Health and Human Services
- Obligated
- $335,098
Award facts
- Assistance listing
- 93.855 Allergy and Infectious Diseases Research
- Completion date 68% complete
- Place of performance
- La Jolla, CA 92093, USA
Award details come from USAspending.gov.
Description
- Abstract
- Nucleus-forming bacteriophages (phages) are a recently discovered class of lytic phages that protect their genome by encapsulating IT in a nucleus-like protein shell (phage nucleus). The goal of the proposed research is to determine whether the unique properties of nucleus-forming phages make them advantageous therapies for human pseudomonas aeruginosa infections. FKZ and FPA3 are nucleus-forming phages that infect p. Aeruginosa and demonstrate the ability to selectively localize proteins to their phage nucleus and exclude others, including DNA-targeting bacterial phage defense proteins. Preliminary data show that FKZ GP69 is a conserved protein in p. Aeruginosa nucleus-forming phages and plays a key role in mediating selective protein trafficking into the phage nucleus. This research will determine the structure of GP69 in complex with other nuclear shell-associated proteins, the mechanism by which GP69 mediates protein transport into the phage nucleus, and other factors that facilitate transport into the phage nucleus. Furthermore, this research will determine the motifs that signal a protein to be imported into the phage nucleus, how those motifs differ between p. Aeruginosa infecting phage, and the competitive advantages selective protein import imparts on nucleus-forming phages. Lastly, resistance to phages is known to select for costly evolutionary trade-offs in the host bacterium. This research will determine how pathogenicity and antibiotic sensitivity are altered in p. Aeruginosa resistant to nucleus-forming phages. Together, the results of this study will provide crucial information for the development of nucleus-forming phage therapeutics.
Transactions
| Action | Obligated | Date |
|---|---|---|
| Amendment | $576 | |
| Amendment | $43,120 | |
| Amendment | $43,120 | |
| Amendment | $564 | |
| Amendment | $564 | |
| Amendment | $564 | |
| Amendment | $42,100 | |
| Amendment | $1,280 | |
| Amendment | $1,280 | |
| Amendment | $0 | |
| Amendment | $0 | |
| Amendment | $0 | |
| Amendment | $0 | |
| Amendment | $0 | |
| Amendment | $0 | |
| Amendment | $0 | |
| Amendment | $40,386 | |
| Amendment | $40,386 | |
| Amendment | $40,386 | |
| Amendment | $40,386 | |
| Original award | $40,386 |
Show all 21
Quick answers
Who received award F30AI176696?
F30AI176696 was awarded to University Of California, San Diego by National Institute of Allergy and Infectious Diseases (National Institutes of Health) on June 6, 2024.
How much is award F30AI176696 worth?
$335,098 has been obligated on F30AI176696.
On GovTribe
See this award's full record
- Every transaction and subaward
- The recipient's other awards and teaming partners
- Similar grant awards
- Ask GovTribe AI about this award