The National Institutes of Health Office of the Director awarded The Trustees of Princeton University a $2,877,560 project grant under the Trans-NIH Research Support program (CFDA 93.310) to build a systematic, comprehensive mammalian cell fate map from September 1, 2022 to August 31, 2025. Leveraging single-cell RNA sequencing and CRISPR-Cas9 genome editing, Princeton University will establish an evolving lineage tracing platform capable of recording mammalian differentiation processes at single-cell resolution. Simultaneously capturing single cell transcriptomes and lineage information will facilitate linking a cell's current state to its developmental history. The University will apply this platform to mouse gastruloid models of embryogenesis to discover differentiation pathways stemming from pluripotency. A hidden Markov model will integrate data to identify trajectories, showcasing multiple potential fates for a given cell type. Findings may inform genetic regulator identification and in vitro cell type manipulation.
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