Project Grant DP2AI192737

Award Date 7/23/25
Completion Date 6/30/30
Dollars Obligated $534K
Federal Grant Program
93.855
Assistance Type
Project Grant
Place of Performance
Pensilvania, EE. UU.
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This $534,000 Project Grant award from the National Institute of Allergy and Infectious Diseases (CFDA 93.855 - Allergy and Infectious Diseases Research) supports research to develop wastewater surveillance systems for monitoring multidrug-resistant bacteria (MDRB). The key objectives are to:

  1. Establish standardized wastewater surveillance strategies to inform clinical infection control responses, using long-read metagenomics and novel bioinformatics to rapidly identify significant changes in MDRB levels compared to site-specific baselines.

  2. Identify environmental factors enabling the emergence of novel extended-spectrum beta-lactam- and carbapenem-resistant Enterobacterales (ESBL-E and CRE) genotypes in wastewater, using a novel metagenomic approach to link bacterial hosts with mobile resistance elements.

  3. Devise wastewater testing methodologies suited for resource-limited clinical settings, reducing per-sample costs and analytical burdens, as demonstrated through a pilot at a pediatric hospital in Botswana.

The project, led by The Children's Hospital of Philadelphia, aims to significantly expand the ability of wastewater surveillance to inform hospital and clinical efforts in mitigating the spread of antimicrobial resistance globally.

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