Federal Project Grant Award Summary

The University of Central Florida Board of Trustees received a $272,112 project grant award from the National Science Foundation (NSF) Division of Chemical, Bioengineering, Environmental, and Transport Systems under the Engineering program (CFDA 47.041), effective February 15, 2026, with completion targeted for January 31, 2028. The award funds development and validation of a DNA-based nanostructure termed the "DNA Cephalopod," designed to enhance biosensor response rates and sensitivity. The nanostructure employs flexible DNA tentacles with analyte-binding fragments to preconcentrate target molecules near sensing surfaces, thereby accelerating detection kinetics and improving limits of detection (LOD). This approach addresses fundamental mass transfer limitations that slow conventional biosensor operations.

The project pursues two primary research objectives: (1) designing and optimizing the DNA Cephalopod using split light-up aptamer sensors for homogeneous detection of nucleic acids, with performance evaluation through response kinetics testing and single-nucleotide variant discrimination using pathogen-derived targets; and (2) extending the nanostructure strategy to heterogeneous sensing formats by immobilizing optimized structures on silica microparticles to assess diffusion limitation mitigation. The research deliverables will advance applications in pathogen monitoring, point-of-care diagnostics, and genetic mutation screening while providing workforce development through training of undergraduate and graduate students in biotechnology and biosensor engineering.