The National Science Foundation (NSF) awarded a $299,772 EAGER (Early-concept Grants for Exploratory Research) grant under the Biological Sciences program (CFDA 47.074) to Washington State University (WSU) to develop new molecular tools for identifying protein-protein interactions within specific cellular compartments in plant cells. The project, titled "TOOLS4CELLS: Bimolecular Proximity Tagging", aims to close the gap in existing methods by creating split enzyme versions that can map dynamic protein interactions in different subcellular locations. The research program will leverage bioinformatics, modular cloning, and in-planta activity assays to produce split proximity tagging tools with no background activity when expressed alone, but efficient and specific functionality once the enzyme activity is reconstructed. While the project focuses on plant systems, the developed tools are expected to have versatility across diverse biological applications. The award period runs from May 1, 2024 to April 30, 2026, and there are no planned subawards under this grant.
Mod # | Description | Reason For Modification | Federal Obligation (Click to sort descending) | Date (Click to sort ascending) |
|---|---|---|---|---|
| Not listed | $299.8k | 5/28/24 |