Project Grant 2339464
- This Project Grant award from the National Science Foundation (NSF) Division of Molecular and Cellular Biosciences under the agency's Biological Sciences program (CFDA 47.074) provides $680,000 from August 1, 2023 to July 31, 2026 to the University of Chicago. The project aims to understand the sequences in pre-mRNA that determine which exons are removed during RNA splicing, a critical process in eukaryotic cells for generating mature messenger RNAs. The research will use high-throughput...
- This National Science Foundation (NSF) Project Grant award under the Biological Sciences program (CFDA 47.074) provides $522,131.00 to New York University (NYU) from August 1, 2023 to July 31, 2026. The funded research aims to understand the relationship between RNA sequence features and splicing outcomes, as well as the role that nuclear speckles play in RNA splicing decisions. The project will leverage high-throughput assays and interpretable machine learning to identify sequence-encoded...
- The National Science Foundation (NSF), through its Mathematical and Physical Sciences (CFDA 47.049) federal grant program, awarded a $500,000 Project Grant to the University of California, Irvine (UCI) to investigate splicing, a fundamental cellular process that produces multiple distinct proteins from the same gene. This research aims to uncover the rules governing RNA splicing dynamics, which are essential for healthy development but linked to diseases like cancer and neurodegeneration. The...
- This $324,118 National Science Foundation project grant supports research to decipher RNA-based regulatory logic through interpretable machine learning models. Funded under the Mathematical and Physical Sciences program (CFDA 47.049), the three-year award to New York University will develop and apply interpretable neural networks to massive parallel reporter assay datasets. Specifically, the models aim to elucidate the splicing code that determines exon skipping and the 5'UTR code regulating...
- This $240,000 Project Grant award from the National Science Foundation (NSF) Biological Sciences program (CFDA 47.074) funds a postdoctoral research fellowship to investigate the role of genome organization on de novo gene origination. The research aims to test a new theoretical model explaining the process of new gene evolution, by examining how the genome's structural features influence the birth of genes that arise from non-coding regions. The key components of the research include: 1)...
- This Project Grant award from the National Institute of General Medical Sciences (NIGMS) Biomedical Research and Research Training program (CFDA 93.859) will fund the development of computational methods to characterize alternative splicing and genetic determinants from heterogeneous sequence data. The $363,392 award to The Johns Hopkins University will support research efforts to create a statistical framework and specialized tools for differential splicing analysis from various sequencing...
- This Project Grant award from the National Science Foundation (NSF) Division of Molecular and Cellular Biosciences, under the Biological Sciences CFDA program (47.074), will investigate a novel regulatory mechanism called "RNA buffering" that maintains messenger RNA (mRNA) levels despite changes in synthesis and decay rates. The $749,978 project at the University of Utah will explore the protein-protein and protein-RNA interactions involved in this process, which challenges...
- The National Science Foundation (NSF) awarded a $1,199,997 Project Grant under its Biological Sciences program (CFDA 47.074) to the University of Colorado-Denver. The grant will fund research to improve understanding of the mechanisms underlying messenger RNA (mRNA) localization and translation, and develop machine learning models to better predict protein production from mRNA. The project will apply recent advancements in RNA sequencing technology to study how factors like tRNA abundance and...
- This three-year, $275,000 National Science Foundation Division of Chemistry Project Grant will support research into deciphering RNA-based regulatory logic using interpretable machine learning approaches. The awardee, Cornell University, will collaborate with researchers to design and deploy interpretable neural networks trained on massively parallel reporter assays. This will provide insights into splicing codes that determine exon skipping and the role of 5'UTR sequences in translation...
- This Project Grant award from the National Science Foundation's (NSF) Integrative Activities program (CFDA 47.083) provides $179,978.00 to South Dakota State University to conduct research on the mechanistic regulation of dynamic gene expression patterns by temporal JNK activation. The project aims to expand understanding of how cells process information and respond to stress, with a focus on two key mechanisms: post-transcriptional regulation of genes through mRNA stability and combinatorial...
CAREER: UNCOVERING THE ROLE OF SPLICING FACTORS IN TRANSCRIPTIONAL REGULATION -IN THE INTRICATE PROCESS OF GENE EXPRESSION, WHICH BEGINS WITH TRANSCRIPTION, RNA UNDERGOES A SELECTIVE EDITING PROCESS CALLED SPLICING IN WHICH SOME PARTS ARE RETAINED, WHILE OTHERS ARE DISCARDED. TRANSCRIPTION AND SPLICING ARE LINKED IN TIME AND SPACE. DESPITE THEIR CONNECTION, THE EXACT MOLECULAR MECHANISMS GOVERNING THEIR COUPLING REMAIN ELUSIVE. THIS PROJECT WILL INVESTIGATE THE MECHANISM UNDERLYING THE FEEDBACK OF SPLICING ON TRANSCRIPTIONAL REGULATION, FOCUSING ON THE ROLE OF THE SPLICING FACTOR U1 SMALL NUCLEAR RIBONUCLEOPROTEIN PARTICLE (U1SNRNP). BEYOND THE LAB, THIS INITIATIVE EXTENDS ITS REACH TO HIGH-SCHOOL AND MIDDLE-SCHOOL STUDENTS AND EDUCATORS, PRIORITIZING OUTREACH TO THE HISPANIC POPULATION. BY NURTURING SCIENTIFIC CURIOSITY AND LITERACY, THE RESEARCHERS WILL OFFER HANDS-ON WORKSHOPS, LAB INTERNSHIPS, AND TUTORIALS ON COMPUTATIONAL BIOLOGY AND STATISTICS. OVERALL, THIS PROJECT NOT ONLY PROMISES TO UNVEIL NOVEL LAYERS OF GENE EXPRESSION REGULATION BUT ALSO ACTIVELY CONTRIBUTES TO CULTIVATING THE NEXT GENERATION OF SCIENTISTS, FOSTERING INCLUSIVITY IN STEM EDUCATION. TRANSCRIPTION AND SPLICING ARE INTRINSICALLY COUPLED; HOWEVER, THE MOLECULAR MECHANISMS UNDERLYING THEIR CO-REGULATION REMAINS POORLY UNDERSTOOD. THIS PROJECT, CENTERING ON THE U1 SMALL NUCLEAR RIBONUCLEOPROTEIN PARTICLE (U1SNRNP), AIMS TO UNVEIL THE MODES OF ACTION AND PRINCIPLES GOVERNING THE FEEDBACK FROM SPLICING TO TRANSCRIPTIONAL REGULATION. THE STUDY IS STRUCTURED INTO TWO MAIN AIMS: (I) DETERMINING THE RULES DICTATING U1SNRNP-DEPENDENT PROMOTER REGULATION THROUGH SPLICING, AND (II) EXPLORING THE MECHANISMS DRIVING U1SNRNP-DEPENDENT DOWNSTREAM PROMOTER ACTIVATION VIA TRANSCRIPT LENGTH REGULATION. THE FIRST AIM INVOLVES ASSESSING THE DEPENDENCY OF U1SNRNP-MEDIATED PROMOTER REGULATION ON THE STRENGTH AND LOCATION OF 5' SPLICE SITES. IN THE SECOND AIM, THE PROJECT DELVES INTO THE EFFECTS OF U1SNRNP INHIBITION ON GLOBAL ALTERNATIVE PROMOTER USAGE, CHROMATIN STRUCTURE, AND PROMOTER DIRECTIONALITY. BY UNRAVELING THE MECHANISMS BEHIND THE ROLE OF U1SNRNP IN PROMOTER REGULATION, THIS PROJECT WILL REVEAL NOVEL LAYERS OF GENE REGULATION. ULTIMATELY, THE FINDINGS OF THIS PROJECT WILL PAVE THE WAY FOR THE DEVELOPMENT OF ADVANCED COMPUTATIONAL TOOLS CAPABLE OF PREDICTING GENE REGULATORY NETWORKS AND DESIGNING THERAPEUTIC MOLECULES FOR PRECISE CONTROL OF GENE EXPRESSION. THIS AWARD REFLECTS NSF'S STATUTORY MISSION AND HAS BEEN DEEMED WORTHY OF SUPPORT THROUGH EVALUATION USING THE FOUNDATION'S INTELLECTUAL MERIT AND BROADER IMPACTS REVIEW CRITERIA.- SUBAWARDS ARE NOT PLANNED FOR THIS AWARD.
Mod # | Description | ReasonForModification | Federal Obligation | Date |
|---|---|---|---|---|
| Not listed | $260.0k | 8/15/25 | ||
| Not listed | $260.0k | 4/15/25 | ||
| Not listed | $260.0k | 2/7/24 |