Project Grant 2325415

Award Date 9/1/23
Completion Date 8/31/27
Dollars Obligated $400K
Federal Grant Program
47.074
Assistance Type
Project Grant
Place of Performance
Buffalo, NY 14203, USA
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The Research Foundation for the State University of New York (SUNY) received a $400,000 Project Grant from the National Science Foundation (NSF) Division of Molecular and Cellular Biosciences under CFDA 47.074 - Biological Sciences. The project aims to improve understanding of the DNA mismatch repair (MMR) pathway and how changes in the behavior of mismatch repair proteins can lead to genome instability, particularly in the presence of specific DNA sequences like repetitive elements. Key project activities include:

  • Identifying allosteric changes in the MSH2-MSH3 protein complex when bound to MMR versus triplet nucleotide repeat (TNR) DNA structures using cross-linking mass spectrometry
  • Determining the interaction interfaces between MSH2-MSH3 and downstream MLH protein complexes in the presence of MMR or TNR DNA structures using genetic approaches
  • Training undergraduate and graduate students in genetics, molecular biology, and biochemical techniques related to the research
  • Engaging high school students from the Buffalo Public Schools, which has a student population that is ~80% Black, Indigenous or people of color, to promote STEM education and awareness of science careers
  • Conducting classroom workshops and public science events like Genome Day to reach K-12 students and the general public

The project will run from September 1, 2023 to August 31, 2027. The outcomes are expected to contribute to the understanding of MMR initiation, signaling, and how it can lead to genome instability in the presence of alternative DNA structures.

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