The National Science Foundation awarded a $179,150 Project Grant to the Donald Danforth Plant Science Center under the Biological Sciences federal grant program (CFDA 47.074) for the period of June 15, 2022 through May 31, 2024. The award will support the development of new computational tools and methods to translate single-cell RNA sequencing data into an ultra-high resolution 3D spatial map of gene expression in plant roots. Specifically, the grantee will leverage existing fluorescent reporter gene lines in Arabidopsis to perform proof-of-concept studies combining single-cell RNA sequencing with fluorescent imaging and semantic modeling. The goal is to establish a new approach for high-resolution spatial transcriptomics in plants that maps gene expression from individual cells within a 3D root model. Results from this research aim to provide new insights into asymmetrical gene expression during plant development and stress responses.
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