**AWARDS ISSUED PRIOR TO JANUARY 20, 2025, WERE FUNDED UNDER PREVIOUS ADMINISTRATIONS AND MAY NOT REFLECT THE PRIORITIES AND POLICIES OF THE CURRENT ADMINISTRATION.** INEFFECTIVE USE OF HERBICIDES TO MANAGE MULTIPLE HERBICIDE RESISTANT WEEDS NEGATIVELY IMPACTS CROP PRODUCTIVITY, FARMERS' INCOMES, HUMAN WELLBEING, AND ENVIRONMENTAL HEALTH. PREVENTING UNPRODUCTIVE HERBICIDE APPLICATION IS AN IMPORTANT ASPECT OF INTEGRATED PEST MANAGEMENT. WE PROPOSE TO DEVELOP TECHNOLOGY FOR DETECTING MULTIPLE HERBICIDE RESISTANT WEEDS PRIOR TO HERBICIDE APPLICATION, ENABLING PRODUCERS TO STRATEGICALLY UTILIZE ALTERNATIVE MANAGEMENT PRACTICES THUS REDUCING THE AMOUNT AND FREQUENCY OF HERBICIDE APPLICATION. WE ARE USING THE GLOBAL WEED PEST AVENA FATUA L. (WILD OAT) IN CEREALPRODUCTION AS THE TARGET WEED AND CROPPING SYSTEM FOR TECHNOLOGY DEVELOPMENT. OUR PRELIMINARY DATA SHOWS PIGMENT AND HYPERSPECTRAL DIFFERENCES BETWEEN HERBICIDE SUSCEPTIBLE AND RESISTANT WILD OAT LINES, SO WE HAVE STRUCTURED OUR OBJECTIVES TO TRANSFORM THOSE BASIC FINDINGS INTO ACTIONABLE TECHNOLOGIES. WE WILL DETERMINE THE RELATIONSHIPS BETWEEN HERBICIDE RESISTANCE AND PLANT COLORATION, PIGMENT LEVELS, AND SPECTRA TO UNDERSTAND WHICH METRICS CAN BE ACCURATELY LEVERAGED TO DIFFERENTIATE HERBICIDE-RESISTANT FROM -SUSCEPTIBLE LINES. WE WILL ASSESS THE SENSITIVITY OF THESE METRICS TO VARIOUS LOCATIONS, INCLUDING ON-FARM. WE WILL COMMUNICATE FINDINGS WITH STAKEHOLDERS THROUGH EXTENSION ACTIVITIES AND SOLICIT FEEDBACK TO STRENGTHEN TRANSLATING FINDINGS INTO DESIRED TOOLS.