This firm-fixed-price purchase order was awarded by the National Institute on Aging (NIA), a civilian agency, to The Johns Hopkins University for $358,152.00. The contract is for performing mouse whole genome bisulfite sequencing, bulk RNA sequencing to support immune cell gene regulation research, and single-cell RNA sequencing to support Alzheimer's disease-related dementias research. The period of performance is from September 2020 to September 2022. This contract was not set aside and was competed openly. The Johns Hopkins University is a private, non-profit research university with extensive federal research and contracting capabilities, including work for the Department of Defense, NASA, and DARPA.