Multiple year award and incremental funding for years one to three. This proposal is entitled, "reliving the past: experimental evolution of major transitions in the history of life - GIT." this is…

This is a multiple-year, incrementally funded grant awarded by the National Aeronautics and Space Administration (NASA) to the Georgia Tech Research Corporation (GTC). The grant, valued at $5,540,143.87, is in response to the NASA Astrobiology Institute's Cooperative Agreement Notice (CAN) and will support a project titled "Reliving the Past: Experimental Evolution of Major Transitions in the History of Life - GIT." The principal investigator is Dr. Raphael F. Rosenzweig from the Georgia Institute of Technology. The project aims to study the forces that drive major transitions in the evolution of biological complexity, focusing on topics such as the evolution of enzymes and metabolic networks, the origins of the eukaryotic cell, the evolution of symbioses, the emergence of multicellularity, and the roles of pleiotropy, epistasis, and mutation rate in the evolution of novel traits. This grant does not have a set-aside designation and is expected to be completed by December 31, 2021.

Multiple year award and incremental funding for years one to three. This proposal is entitled, "reliving the past: experimental evolution of major transitions in the history of life - GIT." this is awarded in response to cooperative agreement notice (can) no. NNH13ZDA017C, NASA astrobiology institute, cycle seven (7). The principal investigator is DR. Raphael f. Rosenzweig with georgia tech research corporation (GIT). Project summary: to illuminate the laws that produce darwin's "tangled bank" remains one of biology's grand challenges, one that requires understanding how differences amount forms are selected for and how interdependence amount forms is enforced. In natural environments, meeting this challenge is complicated by the fact that selection pressures often vary widely over space and through time. Laboratory evolution experiments with microbial population offer an attractive alternative by which to study, under controlled conditions, both the dynamic interplay between genotype and phenotype and the interactions among phenotypes in simple communities. Indeed, because the principal actors in the first 2.5 billion years of life's drama were exclusively microbes, we are obligated to use these forms to unlock the secrets of life's transitions from simple autocatalytic units to self-organizing, self-replicating, energy transduction systems that range in complexity from single cells to ecosystems. Our team is responding to NAI can 7 with a multidisciplinary proposal to address: what forces bring about major transitions in the evolution of BIO-complexity? Our program aims to meet the grant challenge of illuminating and interpreting the laws that produce the "tangled bank" so that we may better understand and appreciate the grandeur in the view of life....that, whilst this planet has gone cycling on according to the fixed law of gravity, from so simple a beginning endless forms most beautiful and most wonderful have been, and are being, evolved. The laws original inferred by darwin were those that give rise to natural selection, which is a consequence of natural variation, inheritance of variation, and the differential reproduction and survivorship of variants according to their fitness. 150 year later we now recognize the importance of other factors, in particular change, whether in the form of genetic drift or historical contingency. Our knowledge of the mechanism of inheritance continues to be refined by new insights into what constitutes a gene, how genes interact and how mutation rate itself is subject to natural selection. Our astrobiology project is organized around a set of questions related to major transitions in the history of life, when independently replicating entities combine in to larger, more complex wholes: (i) how do enzymes and metabolic networks evolve?, (II) how did the eukaryotic cell, specifically the cell that contained a mitochondrion, come to be?, (III) how do symbioses arise?, (IV) how does multicellularity evolve?, and (v) how do pleiotropy, epistasis and mutation rate constrain the evolution of novel traits? A unifying them underlying these questions is: how do cooperative VS. Competitive interactions play out in driving major transitions? Seeking the answers to these questions falls squarely within astrobiology, which is the study of the origins, evolution, distribution, and future of life in the universe, and helps to address the first of our discipline's three principal questions: how does life begin and evolve?