RNA Extraction and Sequencing
Closed Solicitation Posted
This opportunity was awarded. See the award notice from .
- Solicitation number
- 1232SA25Q0142
- Agency
- Agricultural Research Service Department of Agriculture
- Responses due
- Set-aside
- Total Small Business
Opportunity facts
- NAICS code
- 541380 Testing Laboratories and Services
- Place of performance
- Kearneysville, West Virginia 25430, United States
- Points of contact
-
- Christopher Turner christopher.c.turner@usda.gov (970) 851-3723
Notice details come from SAM.gov. Updated .
About this opportunity
The United States Department of Agriculture's Agricultural Research Service is seeking RNA extraction and sequencing services for 120 frozen rainbow trout whole-body tissue samples through a competitive solicitation. The Request for Quotation (RFQ) 1232SA25Q0142 requires contractors to extract total RNA meeting specific quality standards, including a minimum concentration of 250ng/uL and RNA Integrity Number greater than 7. Contractors must also prepare Illumina RNA-seq libraries with paired-end 150 cycle sequencing, generating a minimum of 40 million paired-end reads per sample. The procurement will be evaluated using a Lowest Priced Technically Acceptable (LPTA) method, with technical acceptability determined by responsiveness to solicitation requirements and compliance with submission instructions. Quotations must be submitted via email to Christopher Turner by June 15, 2025, with all deliverables to be completed within 30 days of receiving samples.
The solicitation is a total small business set-aside under NAICS code 541380, with a small business size standard of $19 million. The place of performance is the NEA Cool and Cold Water Aquaculture facility located at 11861 Leetown Road in Kearneysville, West Virginia 25430. The period of performance is 90 days after receipt of order, with a 60-day timeline for sample processing after contract award. Contractors must be registered in sam.gov and comply with all Federal Acquisition Regulation (FAR) clauses and provisions. The contract will be awarded as a commercial services acquisition, with quotes expected to remain valid for 30 days and payment to be made in arrears upon successful completion of tasks as defined in the Quality Assurance Surveillance Plan. The government requires 100% compliance with specified performance standards throughout the contract execution.
Notice text
04JUN25 Base posting. Any amendment and document updates related to this solicitation will be available electronically at the Government Point of Entry (sam.gov). It is the offeror's responsibility to monitor sam.gov Contract Opportunities regarding this solicitation.
(i)This is a combined synopsis/solicitation for a commercial service prepared in accordance with the format in FAR subpart 12.6, as supplemented with additional information included in this notice. This announcement constitutes the only solicitation; quotes are being requested and a written solicitation will not be issued.
(ii)The solicitation number is 1232SA25Q0142 and is issued as a request for quotation (RFQ).
(iii)This solicitation document incorporates those provisions and clauses in effect through Federal Acquisition Circular 2025-03.
(iv)Set Aside: Total Small Business. NAICS Code: 541380 Small Business Size Standard: $19 million
(v) See clauses and provisions document section “SCHEDULE and CLIN Structure” for line item number(s), quantity, and unit of measure.
(vi)The USDA, Agricultural Research Service requires RNA Extraction and Sequencing. See “Attachment 001 Performance Work Statement and QASP” and other attachments for more information.
(vii)Dates: See clauses and provisions document “GENERAL INFORMATION” section for Period of Performance data
Place of Delivery and Acceptance:
NEA COOL AND COLD WATER AQUACULTURE
11861 LEETOWN ROAD
KEARNEYSVILLE WV 25430
FOB Point: Destination
(viii)The provision at 52.212-1, Instructions to Offerors-Commercial Products and Commercial Services, applies to this acquisition. See “Instructions for the Preparation of Offers (FAR 52.212-1 Addendum)” in the clauses and provisions document.
(ix)The provision at 52.212-2, Evaluation-Commercial Products and Commercial Services is detailed in the clauses and provisions document under “FAR 52.212-2 Evaluation-Commercial Items (Addendum.)”
(x)Online sam.gov completion of the provision at 52.212-3 is advised.
(xi)The clause at 52.212-4, Contract Terms and Conditions-Commercial Products and Commercial Services, applies to this acquisition. Any addenda will be noted in the clauses and provisions document.
(xii)The clause at 52.212-5, Contract Terms and Conditions Required To Implement Statutes or Executive Orders-Commercial Products and Commercial Services, applies to this acquisition. Additional clauses are detailed in the clauses and provisions document.
(xiii)Not applicable.
(xiv)Not applicable.
(xv)All sources wishing to quote shall furnish a quotation by the time and dated noted in the General Information, “Date Offers Due” fields above.
(xvi)Quotations are to be sent via email to Christopher Turner at christopher.c.turner@usda.gov.
Notes:
Solicitation Inquiries:
a. Inquiries and all correspondence concerning this solicitation prior to contract award shall be submitted in writing only to the Contracting Officer.
b. Inquiries shall be via Email only, no phone inquiries will be accepted.
c. The Contracting Officer’s email contact information is listed as the PRIMARY POINT OF CONTACT in the sam.gov Contract Opportunities posting.
Time Zones: Please note time zone designations in the "General Information" section above. Other time zones are as designated in the solicitation attachments.
Failure to provide the required information in the requested format may result in quotes not being considered for award.
Attached Provisions/Clauses apply to this RFQ and any subsequent award.
Attachments
| File | Type | Posted |
|---|---|---|
| Attachment 001 Performance Work Statement and QASP.pdf | ||
| Attachment 002 Wage Determination 2015-4285.pdf | ||
| Attachment 003 Transmittal Summary.pdf | ||
| RFQ 1232SA25Q0142 Clauses and Provisions.pdf |
Notice history
| Notice | Type | Posted |
|---|---|---|
| RNA Extraction and Sequencing - Award Notice | Award Notice | |
| RNA Extraction and Sequencing | Solicitation |
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