Attachment 001 Performance Work Statement and QASP.pdf
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- Attached to
- RNA Extraction and Sequencing Federal contract opportunity
- Solicitation number
- 1232SA25Q0142
About this file
This Performance Work Statement (PWS) and Quality Assurance Surveillance Plan (QASP) details a contract for RNA extraction and mRNA sequencing of 120 frozen rainbow trout whole-body tissue samples for the USDA Agricultural Research Service's National Cool and Cold Water Aquaculture Center. The contractor must extract total RNA with specific quality requirements, including a minimum concentration of 250ng/uL, A260/A280 value between 1.8-2.0, and RNA Integrity Number (RIN) greater than 7. Additional requirements include Illumina RNA-seq library preparation using PE 150 cycles, generating a minimum of 40 million paired-end reads per sample with over 85% of bases having quality score ≥30.
The contract specifies a 60-day timeline for sample processing after contract award, with all deliverables (including RNA sequencing data, remaining RNA samples, and a final project report) to be completed within 30 days of receiving all samples. The solicitation is set aside for small businesses under NAICS code 541380, with a small business size standard of $19 million. Deliverables must be submitted to the USDA-ARS-NCCCWA in Kearneysville, WV, either via secure portal download or external hard drives. The Quality Assurance Surveillance Plan requires 100% compliance with the specified performance standards.
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Other files for this federal contract opportunity
| File | Type | Posted |
|---|---|---|
| RFQ 1232SA25Q0142 Clauses and Provisions.pdf | ||
| Attachment 002 Wage Determination 2015-4285.pdf | ||
| Attachment 003 Transmittal Summary.pdf |
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Text version
PERFORMANCE WORK STATEMENT AND QASP
RNA extraction and Sequencing of 120 Rainbow Trout Samples
Scope of Work: The USDA ARS NCCCWA requires total RNA extraction and mRNA sequencing of 120 frozen samples of rainbow trout whole-body tissue homogenized in
Trizol.
Contractor shall provide all services, supplies, equipment and facilities necessary for total
RNA extraction and mRNA sequencing of 120 frozen samples of rainbow trout whole-body tissue homogenized in Trizol. Contractor shall fully meet the required performance elements and benchmarks described below.
Schedule of Line Items:
Line Item 01 120 Samples
All 120 samples will be provided within 60 days after the contract is awarded.
Required performance elements and benchmarks:
1. Total RNA shall be extracted and quantified by the contractor. The total RNA should be stored in nuclease free water with a minimum concentration >250ng/uL and a minimum volume >200uL.
2. Contractor shall re-extract total RNA until RNA quality for each sample meets the following quality requirements. The A260/A280 value of each RNA sample shall be within a range from 1.8 to 2.0. The RNA quality shall also be evaluated with
BioAnalyer RNA chip or similar, and RIN value for each sample shall be greater than 7.
3. Standard Illumina RNA-seq library preparation shall be performed by the vendor.
4. Platform and configuration: Illumina technology, PE 150 cycles.
5. Read Length: 2 x 150 nucleotides (paired-end)
6. Data Output: A minimum of 40 million paired-end reads per sample (20M reads in each direction). For each read, greater than 85% of bases shall have QC≥30.
The output data files are in standard fastq format.
7. Contractor shall have in place a series of quality control (QC) steps during RNA extraction, library preparation and mRNA sequencing.
8. The remaining total RNA shall be returned in clearly labeled microcentrifuge tubes and shipped overnight with dry ice.
9. Additional deliverables shall include the RNA sequencing data for all samples, and a Final Project Report describing RNA extraction, library preparation, QC procedures and sequencing methods. All data must be provided via secure portal download OR in external hard drives to the USDA-ARS-NCCCWA, 11861
Leetown Road, Kearneysville, WV 25430.
Completion and return of all deliverables are required within 30 days from receipt of all
120 samples. The order number shall be included on all documentation and shipments.
QUALITY ASSURANCE SURVEILLANCE PLAN (QASP). The Government shall establish a QASP as a means of evaluating whether the Contractor is meeting the performance standards/quality levels identified in the PWS to ensure that the government pays only for the level of services received.
Task Standard Acceptable Quality
Level (AQL)
Completion and return of all deliverables as described in the
“Required performance elements and benchmarks” section
Task completed within 30 days from receipt of all 120 samples with order number on all documentation and shipments
100%
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