Protein Analysis Rainbow Trout

The Department of Agriculture Agricultural Research Service (USDA ARS) has issued Solicitation 1232SA26Q0113, a Request for Quotation (RFQ) seeking proteomics analysis services for rainbow trout protein samples. The contractor will perform 4D-DIA (four-dimensional data-independent acquisition) analysis using a timsTOF LC-MS/MS platform on 36 lysosomal protein preparations isolated from rainbow trout liver, divided into eight treatment groups. Key requirements include executing comprehensive sample preparation, constructing a spectral library from pooled samples, and delivering a detailed technical report documenting experimental procedures, instrument parameters, and all identified and quantified protein data formatted for pairwise and multiple comparisons. The USDA will evaluate proposals using the lowest price technically acceptable (LPTA) methodology, with only the lowest-priced offer initially assessed for technical acceptability and past performance; both technical acceptability and past performance must receive an "acceptable" rating for consideration. Critical deadlines include January 26, 2026, at 12:00 PM Mountain Time for submission of questions to amber.sampson@usda.gov, February 2, 2026, for proposal submission, and March 1, 2026, for delivery of all completed work and deliverables.

This acquisition is designated as a Total Small Business Set-Aside with a small business size standard of $19 million, limiting competition to small business concerns. The contract is structured as a firm-fixed-price award with a maximum performance duration of 60 days from sample receipt, and the USDA intends to make a single award to one contractor. The specific numerical requirement consists of 36 protein samples at 50 micrograms of protein per sample, with the USDA delivering samples within two weeks of award and expecting all data deliverables, including an Excel spreadsheet with results and technical documentation, within 60 days of sample receipt. The place of performance for sample delivery and the contractor's primary work location is Kearneysville, West Virginia 25430, with all finished deliverables to be shipped to USDA-NCCCWA at 11861 Leetown Road, Kearneysville, WV 25430-3804. Offerors must submit comprehensive pricing for all requirements, hold prices firm for 60 calendar days following the solicitation closing date, and provide supporting documentation demonstrating their technical capability and past performance on similar proteomics analysis projects.