A.01.13_PWS_Att4_Water_Qual.pdf

ATTACHMENT 3

DWORSHAK NUTRIENT ENHANCEMENT PROJECT

WATER QUALITY ANALYSIS

1.0 Field Collection – For Information Only (FIO)

1.1 Sampling Design. Personnel from IDFG will collect the field data and send water and biological samples for analysis. Seven limnologic stations will be sampled during the application season; six throughout Dworshak Reservoir and a single station in the North Fork Clearwater River below Dworshak Dam (NFC), as described in the diagram and table below.

Map of Dworshak Reservoir, major tributaries, reservoir sections, and limnologic sampling stations, North Fork Clearwater River, Idaho.

River Kilometer

River Mile

Reservoir Zone Location Description

NFC 2.5 0 In North Fork Clearwater (NFC) below Dworshak Dam at IDFG boat ramp

RK-2 3 1 At USACE water quality buoy

EC6 EC 4 1 At no wake buoy in Elk Creek (EC) arm

RK-31 19 2 At USACE water quality buoy downstream of Cranberry Creek

RK-56 35 2 At USACE water quality buoy between Silver and Gold Creeks

LNF-3 LNF 2 3 At USACE water quality buoy near the mouth of Gleason Creek on the Little North Fork (LNF)

RK-72 45 3 At USACE water quality buoy between Benton and Anderson Creeks

LNF-3

NFC

RK-31

Zone 1

Zone 2

Zone 3

RK-56

RK-2

EC-6

RK-72

1.2 Sampling Schedule. Since sampling all stations in the same day may not be possible, two consecutive days may be needed to complete all stations, which comprise a single “sample event.” The table below indicates the sampling schedule and number of samples expected by parameter. Dates are approximate and are subject to change. There will be one sampling event each month April through November.

Sampling schedule and number of samples expected by parameter (dates approximate).

Sampling Water Chemistry* Biologic Analysis** Dates (approx.) TP TDP TN TAN NOX TDS DOC Chl a Pico Phyto Zoop Apr 10-11 11 11 11 11 11 10 2 8 6 6 6 May 8-9 11 11 11 11 11 10 2 8 6 6 6 Jun 12-13 11 11 11 11 11 10 2 8 6 6 6 Jul 10-11 11 11 11 11 11 10 2 8 6 6 6 Aug 14-15 11 11 11 11 11 10 2 8 6 6 6 Sep 11-12 11 11 11 11 11 10 2 8 6 6 6 Oct 10-11 11 11 11 11 11 10 2 8 6 6 6 Nov 13-14 11 11 11 11 11 10 2 8 6 6 6 Project Total 88 88 88 88 88 80 16 64 48 48 48

*TP=Total Phosphorus; TDP=Total Dissolved Phosphorus; TN=Total Nitrogen; TAN=Total Ammonia Nitrogen;

NOX=Nitrate+Nitrite Nitrogen; TDS=Total Dissolved Solids; DOC=Dissolved Organic Carbon;

**Chl a=Chlorophyll a; Pico =Picoplankton; Phyto=Phytoplankton; Zoop=Zooplankton

1.3 Sampling for Blue-green Algae Blooms. Ad hoc phytoplankton samples will be collected as needed in accordance with NPDES Permit ID-0028444 for analyzing Blue-green Algae blooms, should they occur. Samples may be taken anywhere along the reach of the reservoir and the North Fork Clearwater River and tributaries.

1.4 Monitoring Program Methods. Monitoring program methods are included in the report “Dworshak Reservoir:

Rationale for Nutrient Supplementation for Fisheries Enhancement” and the “Sampling and Analysis Plan (SAP) and Quality Assurance Project Plan (QAPP) for Dworshak Reservoir Nutrient Enhancement Project.” These documents will be provided upon request.

2.0 Laboratory Analyses

2.1 Analytical Methods Requirements. Procedures for laboratory analysis will be in accordance with procedures acceptable to both IDFG and USACE. Laboratory SOPs must be consistent with requirements as specified in the method. Copies of laboratory SOPs are retained by the contract labs and are to be available for review by IDFG, USACE, and USEPA.

2.1.1 Water Chemistry. Total phosphorus (TP), total dissolved phosphorus (TDP), nitrate + nitrite (NOX), total nitrogen (TN), and total ammonia (TAN) parameters must be detected to as “low level” as possible. This should be 0.001 mg/L for TP, TDP, and NOX, and 0.010 mg/L for TN, and 0.005 mg/L for TA, which will be specified again in the chain of custody. For each sampling event, the samples will be analyzed by the methods presented in the table below, along with total dissolved solids (TDS) and dissolved organic carbon (DOC).

Analytical program summary for water samples – Water Chemistry

Analyte Analytical

Method

Method Detection Limit (mg/l)

(Contract Lab)

Practical Quantitation Limit (mg/l)

(Contract Lab) Total Phosphorus SM 4500 P E 0.001 0.005

Total Dissolved Phosphorus SM 4500 P E 0.001 0.005 Total Nitrogen SM 4500 N C 0.010 0.050 Total Ammonia EPA 350.1 0.005 0.015 Nitrite+Nitrate EPA 300.0 0.001 0.005

Analyte Analytical

Method

Method Detection Limit (mg/l)

(Contract Lab)

Practical Quantitation Limit (mg/l)

(Contract Lab) Total Dissolved Solids SM 2540 C 1 5

Dissolved Organic Carbon SM 5310 B 0.50 1.5

2.1.2 Biologic Analysis. Laboratory analytical parameters for biological community include chlorophyll a, phytoplankton, pico-cyanobacteria, heterotrophic bacteria, and zooplankton. The samples will be analyzed by the methods presented in the table below. In accordance with NPDES requirements: the Contractor shall report to USACE when chlorophyll a annual median is approaching NPDES Permit limit or is probable to exceed permit limit; and, the Contractor shall report to USACE when Sechhi depth annual median is approaching NPDES permit limit or is probable to exceed permit limit.

Analytical program summary for biological samples – Biologic Analysis

Analyte Analytical Method Chlorophyll a EPA 445.0 Phytoplankton Utermohl (1958) Picoplankton MacIsaac and Stockner (1993)

Heterotrophic Bacteria Porter and Feig (1980) Zooplankton TWRI B-2501-85

2.1.3 Blue-Green Algae. Analytical parameters for analyses and methods are shown in the table below.

Analytical program summary for biological samples – Blue-Green Algae

Description Parameter Results Detection Phytoplankton (Blue-green only) Cells/mL

Toxins Anatoxin A Presence and concentration Microcystin Toxin

2.4 Standards Traceability. All reagents/solutions (media ingredients, positive controls, buffers, etc.) used in the laboratory are traceable to certified reference materials. Each documentation includes information concerning the standard identification, starting materials, including concentration, amount used, and lot number, date prepared, expiration date, and preparer’s initials or signature. The reagent bottle must be labeled in a way that will trace the reagent back to preparation.

2.5 Analytical Method Modification. Only data generated using approved analytical methodologies, as specified in the attached QAPP, will be submitted to IDFG and USACE. Changes to the methodologies specified in this document (modifications of USEPA-approved methods, new methods, etc.) are changes to the QAPP, as described in Section 1.4 of the QAPP. Approval may involve equivalency testing, etc. Amended work will only begin after the IDFG and USACE have approved the modified procedures.

2.6 Failures in Measurement Systems and Corrective Action. Failures in field and laboratory measurement systems involve, but are not limited to, such things as blank contamination, QC samples outside QAPP-defined limits, etc. In many cases, the field technician or lab analyst will be able to correct the problem. If the problem is resolvable by the field technician or lab analyst, then they will document the problem on the field data sheet or laboratory record and complete the analysis. If the problem is not resolvable, then it is conveyed to the IDFG Technical Sampling Lead and USACE Project Manager. If the analytical system failure may compromise the sample results, the resulting data will not be annotated to IDFG or USACE as part of the analysis report.

3.0 Quality Control Requirements

3.1 Quality Control. Each results report must provide a laboratory quality assurance/quality control (QA/QC) review including blanks (LPB), laboratory control samples (LCS), and lab matrix spike/duplicates (MS-D). The results must also include field QA/QC sample results for field duplicates, and blanks reported by Relative Percent Difference (RPD).

3.2 Laboratory Measurement Quality Control Requirements and Acceptability Criteria

3.2.1 Laboratory Quality Assurance. Detailed laboratory QC requirements are contained within each individual method and Laboratory Quality Assurance manuals. The Laboratory Quality Assurance manuals and calibration logs shall be provide to the USACE upon request. The minimum requirements that all participants abide by are stated below. Lab QC sample results are included with the data report.

3.2.2 Laboratory duplicate Laboratory duplicates are used to assess precision. A laboratory duplicate is prepared by splitting aliquots of a single sample (or a matrix spike or a laboratory control standard) in the laboratory.

Both samples are carried through the entire preparation and analytical process. Laboratory duplicates are analyzed on 10% of samples.

3.2.3 Performance limits and control charts are used to determine the acceptability of duplicate analyses.

3.2.4 Method Blank A method blank is an analyte-free matrix to which all reagents are added in the same volumes or proportions as used in the sample processing and which is then analyzed with each batch. The method blank is carried through the complete sample preparation and analytical procedure. The method blank is used to document contamination from the analytical process. The analysis of method blanks should yield values less than the PQL.

3.2.5 MS/MSD Matrix spikes and matrix spike duplicates (MS/MSD) are used to assess sample matrix interferences and analytical errors, as well as to measure the accuracy and precision of the analysis. The MS/MSDs will be collected and analyzed at a rate of 10% of the field samples for each analytical method, except for the biological parameters, or at least one for each analytical batch, whichever frequency is greater.

Known concentrations of analytes are added to environmental samples; the MS or MSD is then processed through the entire analytical procedure and the recovery of the analytes calculated. Results are expressed as percent recovery of the known spiked amount (and RPD for MS/MSD pairs).

4.0 Laboratory Qualifications

4.1 Suggested Laboratories. The laboratories listed below have provided analysis and data consistently during the previous five years of this program. Maintaining consistent sample collection and laboratory analysis is critical for continued adaptive management of the Dworshak Reservoir Nutrient Supplementation program. In order to maintain the ability to use these data for future program management decision, it is preferable to maintain consistency in the analytical laboratories.

Analysis Laboratory POC Email Phone

Water Chemistry AmTest, Inc. Kathleen Fugiel kathyf@amteslab.com 425-885-1664

Biological Analysis Advanced Eco-Solutions Darren Brandt wqpro@yahoo.com 208-660-8733

4.2 Alternate Laboratories. If different laboratories from the previous are subcontracted:

• The proposed laboratories must demonstrate they employ identical techniques to the previous laboratories when analyzing like samples and utilize methods that meet or exceed the Analytical Methods and Limits described in paragraphs 2.2.1 and 2.2.2 above.

• The proposed laboratories must demonstrate five years of experience identifying the taxa that are found in Dworshak Reservoir, and

• The Contractor must arrange a side-by-side comparison between the new and previous laboratories using test samples collected at the same time and location to ascertain that the new lab is reporting results that are consistent with the previous lab.

4.3 Turnaround Timeline. All contract laboratories must be able to complete select analyses and submit a report of complete results with sufficient to allow for adaptive management decisions to be made for the subsequent fertilizer mailto:kathyf@amteslab.com mailto:wqpro@yahoo.com applications, normally less than two weeks. This fast turnaround of sample results is absolutely critical to real-time adjustments of nutrient enrichment rates.