Statement of Work - GC-MS.pdf

US Department of Agriculture (USDA)

Statement of Work

Contents Project

General Information

1.0 Scope of Work

2.0 Background

Contractor Requirements

3.0 Technical Requirements / Tasks

4.0 Government Furnished

5.0 Deliverables / Schedule

6.0 Travel

7.0 Contractor’s Key Personnel

8.0 Security Requirements

9.0 Data Rights

10.0 Section 508 – Electronic and Information Technology Standards

Attachment

Project Gas Chromatograph/Mass Spectrometer (GC/MS)

General Information

1.0 Scope of Work

Vendor is to deliver a fully automated Gas Chromatograph/Mass Spectrometer for the separation and determining mass/fragments for organic compounds.

Features, Components, Accessories, Modules, Software and/or Services to be included:

1) Gas chromatography (GC) Oven

2) Mass Spectrometer (MS) working in Electron Ionization (EI) mode.

3) Autoinjector for liquid samples with carousel/holder for at least 25 sample vials.

4) Computer system with Windows 10 or 11 OS, 16” or larger monitor, qwerty keyboard, and mouse.

5) Software to control the instrument and analyze the data with integrated NIST, Wiley or equivalent Mass-Spectral library.

6) Vacuum pump.

7) Start up operating supplies.

8) Installation and on-site training.

9) Twelve-month warranty.

2.0 Background

The analysis of mixture of compounds with molecular weights (MW) 3<MW<1000, and which are volatile <400 °C can be done with GC-MS. First, a small amount (0.1-10 µL) of solution of the sample is injected in the instrument. Then, a carrier gas carries the compounds through a capillary column. The column is in the GC oven, which maintains a controlled temperature profile during the run. At the exit of the column, the mass spectra of the compounds are collected by the MS. For the operation of the MS, a high vacuum is required. The compounds are identified by their retention times (the time taken to pass through the column) and the mass spectra. The identification is facilitated by data analysis software, which compares the experimental spectra and retention times to database (NIST or Wiley library) of previous ones. Depending on the type of mixtures and the desired analysis, the system needs to be run at different temperature profiles, carrier gas flow rates, types of injection and types of detection. This is controlled by a computer.

The carrier gases used for GC-MS are hydrogen (H2) or helium (He). Hydrogen is cheap but is a fire and explosion hazard. He is more convenient for use in GC-MS but its price fluctuates and there are possibilities of shortages in the future.

Contractor Requirements

3.0 Technical Requirements / Tasks

1) The instrument should use either hydrogen (H2) or helium (He) as a carrier gas.

a) If the instrument uses He as a carrier gas, it must be designed to consume <1 cylinder (8000 L at 1 atm and 25 °C) He per year, when the instrument is used for ≤8h per weekday and idling during the nights and weekends. A set-up with an automatic switch to nitrogen as a make-up gas during idling is acceptable.

b) If the instrument uses H2 as a carrier gas, it must have safety controls to prevent fire or explosion in case of leak.

2) Oven can accommodate one 7” diameter gas column such as: Perkin-Elmer’s N9306312 or Phenomenex’s Zebron ZB- 35 column(s).

3) Oven with a temperature range of 30 °C to at least 400 °C (when ambient t° = 20 °C).

4) Oven can heat at rates up to 40 °C per minute or more (in the 40 °C to 100 °C interval).

5) Retention time repeatability <0.01 min or < 0.02% (using a 30 m Perkin-Elmer N9306312 column or similar).

6) autosampler and ≥25-samples tray (2 mL sample vials). The injection volume can be chosen in the range 0.5-10 µL or wider.

7) The inlet should allow split/splitless injections.

8) The inlet temperature should be controllable between 100 and 400 °C.

8) Mass range for the MS of 3 – 999 Da or wider.

9) Max Scan Rate ≥10,000 u per second

10) Signal-to-Noise (S/N) ratio (measured with 1pg OFN; @ m/z=272 scanning 50 to 300 Da in EI mode): ≥300:1.

11) Working carrier gas flow rate can be 0.5 to 2 mL/min.

12) Includes a vacuum pump with capacity ≥ 50 L/s.

13) The instrument, computer, monitor, keyboard and pump together should fit onto 100”×27” (WxD) bench and be <50” tall.

14) Power requirement: 120 VAC, 60 Hz, single phase.

4.0 Government Furnished

The government will supply a location with suitable instrument requirements (electricity) as needed to operate the instrument.

5.0 Deliverables / Schedule

Key Deliverables

Item No. Deliverable Objective Due 1 Delivery of a fully automated Gas Chromatograph/Mass Spectrometer

Laboratory instrument meets the specifications identified in this Statement of Work

Delivered no later than 120 days after receipt of order

6.0 Travel

N/A

7.0 Contractor’s Key Personnel

N/A

8.0 Security Requirements

All personnel, government and equipment supplier must comply with NCAUR COVID pandemic guidelines – including the possible wearing of masks and maintaining 6-foot social distancing.

9.0 Data Rights

N/A

10.0 Section 508 – Electronic and Information Technology Standards Section 508 guidelines will be adhered to if applicable.

Attachment N/A