Statement of Requirement.pdf

Statement of Requirement

Project: Evaluation of immunogenicity of different vaccine formulations against Shiga toxin-producing Escherichia coli in cattle

GENERAL INFORMATION

1.0 Scope of Work: Cattle are the primary reservoir for STEC bacteria, including

(Shiga toxin-producing Escherichia coli) STEC O157:H7 (O157). O157-colonized cattle remain healthy but are prone to shed these bacteria in their feces, which pose major risks to the contamination of bovine food products. Most human disease outbreaks and/or sporadic cases of infection, therefore, are linked to the consumption of ground meat, milk, contaminated water, and ready-to-eat vegetables. Animal vaccination is one potential method to limit O157 in The goal is to evaluate different vaccine in inducing O157 specific immune responses in cattle that are near slaughter age. As gender can impact immune responses, we typically perform vaccine trials in same sex animals with all animals of the same breed.

2.0 Background: This project requires healthy heifers of approximately 13-17 months of age. All animals need to be of the same breed or breed mix and within 3 months of age to on another. Dairy breed and or mix breed with a dairy breed is preferable for comparisons to other trials. Heifers must be dewormed, free of ring worm, BVDV-free and sound of foot and body. Cattle must be transported to Ames with no other animals. Certificate of Veterinary Inspection required on all animals.

Cattle must arrive by 8/17/2021.

CONTRACTOR REQUIREMENTS

3.0 Technical Requirements/Tasks: This project requires the purchase of 24 Holstein or Holstein/Angus heifers. For this project we require the following of the heifers:

1) Must be BVD-negative

2) Must be dewormed

3) Must be free of ring-worm

4) Must be sound of foot and body

5) Must be 13-17 months old

6) Must be available for delivery on or near August 11-17, 2021

7) Animals must be transported directly to NADC with no other animals in the trailer.

8) Due to the size of the animals they must be transported on 2 separate trailers from the destination of origin. Two trailers are required to comply with the rules and regulations in the Guide for the Care and Use of Agricultural Animals in Research and Teaching. 4th Edition.

9) Trailer sizes should be approximately 40 foot and 34 foot to accommodate the quantity and size of safe transportation of animals.

10) Both trailers must arrive at the same time to Ames animal facility due to coordination of study events and personnel.

11) Must have 24 heifers available with comparable size across the group of the same breed (mixed dairy/beef breed)

12) Certificate of Veterinary Inspection required on all animals.

4.0 Government Furnished: N/A

5.0 Technical Point of Contact: Should there be technical or scheduling questions, please contact Crystal Loving at 515-337-7364

6.0 Delivery Location:

USDA, 1920 Dayton Avenue, Ames, IA 50010