Specifications.pdf

Specifications Project: Whole Slide Image/Scanner (Brightfield and Fluorescent)

GENERAL INFORMATION

1.0 Scope of Work:

Whole Slide Imager/Scanner will be utilized to scan microscope slides that have been stained/labeled for brightfield or fluorescence. These scanned images are utilized for software applications to be seen in publications and Powerpoint presentations.

2.0 Background: Whole Slide Imaging has been done here at NADC since 2011. The present system will no longer be supported as of June 2024. Having whole slide imaging with necessary magnification options for both brightfield and fluorescent work is essential to do image analysis for publications.

CONTRACTOR REQUIREMENTS

3.0 Technical Requirements/Tasks:

1. Whole Slide Imager/Scanner with 6 position nosepiece for objectives

2. Computer and monitor with color camera to run the whole slide imager

3. Automated Slide Loader System for up to 200 slides of various types including

1”x3”, and 2”x3” glass slides

4. Slide Trays to accommodate 180 1”x3” glass slides and 6 2”x3” glass slides

5. Objectives with flexibility to use dry or oil objectives:

a. ACHO 2X Objective Lens, NA0.08, WD 6.2mm

b. UPLXAPO 4X Objective Lens, OFN26.5, NA0.16, WD13mm

c. Plan Apo 20X Objective Lens, OFN26.5, NA 0.80, WD0.6mm

d. UPLXAPO 40X Objective Lens, OFN26.5, NA 0.95, WD0.18mm, W/CC

e. UPLXAPO 100X Oil Objective, OFN 26.5, NA 1.45, WD 0.13mm

6. Automated Immersion Oil Dispenser System

7. Fluorescent Filter Sets

a. DAPI LED Filter set

b. FITC LED Filter Set

c. MCherry LED Filter Set

d. TRITC LED Filter Set

e. Cy5 LED Filter Set

f. Cy7 LED Filter Set

8. Lightsource for fluorescence

a. Illuminator with fly-eye lens,

b. Motorized 8 position mirror turret

c. Motorized filter wheel

d. Widefield light source options

e. 4-line laser combine (405nm, 488nm, 561nm and 638m and scrabler unit

9. Camera:

a. ORCA Fusion, diagonal 21mm 2304x2304 6.5um sCMOS, 31.6fps

(USB3.0) 89 fps (CoaXP), C-mount, incl. USB3.0 card, USB3.1A-microB CBL, power supply, one trigger cable, HCImage Live SW

10. Cleanbench vibration isolation table

a. 900x1500 mm

b. Thin-wall rolling diaphragm

c. Aluminum height control valves

d. Piston travel restraint

e. Built-in leveling feet

f. Ergonomic for seating

g. Retractable casters

11. Air Compressor, Low Noise, 110V to provide 82 psi compressed air for cleanbench vibration isolation table

12. Data and Image Management Software:

a. Software to run database (automatic bar code reading, automatic focus mapping, automatic scanning, automatic stitching, extended focus imaging, image format compatible with existing HALO software analysis including JPEG and TIFF, stepless zooming, screen capture, slide loader control), administrator and installation.

b. Software that can interface directly with existing Indica Lab HALO software for software analysis.

c. Software than can handle different scanners simultaneously.

d. Software that can import data from a previous slide scanner to have all data in one location.

13. Power Cords, Power Supply and UPS

14. In-house installation and training

15. Qty: 1

4.0 Government Furnished: USDA will provide escort of installation and training staff while in USDA Laboratory facility.

5.0 Deliverables/Schedule: Delivered onsite within 90 days of date of award.

Installation and training shall be completed within 120 days of date of award.

6.0 Technical Point of Contact: Should there be technical questions, please contact

Judi Stasko 515-337-7445.

7.0 Place of Delivery: USDA, 1920 Dayton Avenue, Bldg 24, Ames, IA 50010