UNDER_SAT_Sole_Source-FBO.pdf

SOLE SOURCE JUSTIFICATION

FOR FEDERAL PROCUREMENTS UNDER THE SIMPLIFIED ACQUISITION THRESHOLD

(The use of this template is optional and solely for the convenience of the Contracting Officer)

1. Describe the requirement, as well as the total estimated dollar value. Please note that the requirement is the minimum acceptable specification or capability, and is not necessarily the best in the industry. Please note whether any additional follow-on work is anticipated in response to the current proposed effort.

The USDA, ARS, Plum Island Animal Disease Center (PIADC) in Orient Point, NY, requires an additional next generation sequencing (NGS) system, Illumina NextSeq550. Currently we are undergoing large sequencing efforts for PIADCs biorepository characterization for transfer to NBAF. We require that this NGS be compatible with both our analysis software (basespace) that is propriety software, and with hi throughput paired reads, with a low error rate.

2. List the proposed vendor and contact information.

Illumina, Inc 520 Illumina Way San Diego, CA 92122-4616 Product: NextSeq550

3. Describe why the proposed vendor is the only vendor from which the requirement can be obtained. Examples of acceptable justifications include but are not limited to: sole manufacturer and seller, adherence to warranty provisions, urgency, etc. If other vendors can provide similar products or services, describe why they cannot meet our particular requirement. Attach supporting market research documentation.

USDA ARS has an Illumina Blanket Purchase Order (BPA) that was sole sourced however, this item was not listed on it.

Illumina is the only manufacturer and distributor for Illumina NGS technology. This technology is covered by a wide range of patents and cannot be replicated by other vendors. Illumina is only manufacturer and has a patent for the benchmark of NGS, which can do paired end reads, this is important as by pairing reads you can virtually eliminate errors that occur with other technologies. We are currently using a Nextseq500, having an additional machine will allow increased capacity using the same protocols, reagents and the same workflow. Including being compatible with our current analysis software that is patented by Illumina technologies (basespace). Currently Illumina holds all the patents for this technology. Including US patents: Illumina technology is protected by US Patent Nos. 5,964,443;

6,437,109; 7,375,234; 7,763,736; 8,129,542; 8,580,970; 8,877,939; 9,040,256; 9,080,211; 9,085,801; 9,115,396;

9,683,230; 10,041,066; 7,414,116; 7,329,860; 7,589,315; 7,960,685; 8,039,817; 8,071,962; 8,158,926; 8,244,479;

8,315,817; 8,412,467; 8,914,241; 8,965,076; 9,012,022; 9,068,220; 9,121,063; 9,193,996; 9,365,898; 9,410,977;

9,512,422; 9,540,690; 9,752,186; 9,777,325; 9,976,174; 9,994,687;10,005,083; 10,053,730; 10,227,636; In addition we have purchased specialized tools for our NextSeq500 that are required for calibration and set-up that can be utilized on this machine. Tools have to be purchased as the machine will reside in a BSL3 laboratory.

4. FAR Authority: 13.106-1 (b)(1)(i)